SESSION TITLE: Asthma II
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: To study the mechanism of signaling pathway in which Poly-L-arginine (PLA) and lipopolysaccharide (LPS) synergistically activating the release of interleukin-6 (IL-6) and interleukin-8 (IL-8) in NCI-H292 cells.
METHODS: NCI-H292 cells cultured in vitro were grouped into control group, PLA group, LPS group, PLA+LPS group, according to different action time (15min, 30min, 1h, 1.5h, 2h), the phosphorylation of JNK in each group was measured by Western blot, and in each group stimulation for 1h was compared. We separated NCI-H292 cells into control group, PLA group, LPS group, PLA+LPS group, PLA+SP-600125 group, LPS+SP-600125 group, PLA+LPS+SP-600125 group, SP-600125 group, measured the phosphorylation of JNK by Western blot, detected the IL-6 and IL-8 by enzyme-linked immunosorbent assay (ELISA), and adopted the analysis of variance to analyze the experiment results.