Allergy and Airway: Asthma II |

LPS Synergizing With Poly-L-arginine Activate NCI-H292 Cells to Release IL-6 and IL-8 via JNK Signaling Pathway FREE TO VIEW

Xiao Yun Fan, DrPH; Bing Chen, MD; Yu Wen Liu, MD; Ling Ling Zhang, MD; Qiang Wang, MD
Author and Funding Information

The Geriatric Institute of Anhui, The First Affiliated Hospital of Anhui Medical University, He Fei, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A18. doi:10.1016/j.chest.2016.02.020
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: To study the mechanism of signaling pathway in which Poly-L-arginine (PLA) and lipopolysaccharide (LPS) synergistically activating the release of interleukin-6 (IL-6) and interleukin-8 (IL-8) in NCI-H292 cells.

METHODS: NCI-H292 cells cultured in vitro were grouped into control group, PLA group, LPS group, PLA+LPS group, according to different action time (15min, 30min, 1h, 1.5h, 2h), the phosphorylation of JNK in each group was measured by Western blot, and in each group stimulation for 1h was compared. We separated NCI-H292 cells into control group, PLA group, LPS group, PLA+LPS group, PLA+SP-600125 group, LPS+SP-600125 group, PLA+LPS+SP-600125 group, SP-600125 group, measured the phosphorylation of JNK by Western blot, detected the IL-6 and IL-8 by enzyme-linked immunosorbent assay (ELISA), and adopted the analysis of variance to analyze the experiment results.

RESULTS: PLA, LPS and PLA+LPS increased the phosphorylation of JNK in NCI-H292 cells (P<0.05), compared to control group, the most distinct effect of PLA is at 1.5h (P<0.05), LPS or PLA+LPS is at 1h (P<0.01), and there was a synergistic effect between PLA and LPS (P<0.01). PLA or LPS induced the release of IL-6 and IL-8 in NCI-H292 cells (P<0.05), PLA and LPS synergistically induced the release of IL-6 and IL-8 in NCI-H292 cells (P<0.05). JNK was significantly inhibited by SP-600125 which was a specific inhibitor of JNK signaling (P<0.05), and the expression of IL-6 and IL-8 stimulated by PLA, LPS and PLA+LPS were statistically decreased by SP-600125 (P<0.05).

CONCLUSIONS: JNK signal pathway takes part in the release of IL-6 and IL-8 that is stimulated by the synergism of PLA and LPS in NCI-H292 cells.

CLINICAL IMPLICATIONS: Blocking JNK signaling pathway can inhibit the production of IL-6, IL-8 and reduce airway inflammation, which may contribute to providing the basis of looking for drug targets for ease and treatment of bronchial asthma.

DISCLOSURE: The following authors have nothing to disclose: Xiao Yun Fan, Bing Chen, Yu Wen Liu, Ling Ling Zhang, Qiang Wang

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