SESSION TITLE: Asthma I
SESSION TYPE: Original Investigation Poster
PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM
PURPOSE: Asthma is a chronic inflammatory airway disease. There has been growing interest in the microbiome of the respiratory tract which may have a role in the chronic inflammatory process.
METHODS: We recruited 24 non-smoking patients with stable non-severe asthma, 25 severe asthmatics and 15 healthy subjects in Guangzhou. Induced sputum samples were collected for 16S ribosomal RNA analysis. The V3-V5 regions of bacterial 16S rRNA genes were amplified and sequenced by 454 pyrosequencing. Sequences were assigned to bacterial taxa by comparing them with 16s rRNA sequences in the Ribosomal Database Project.