Allergy and Airway: Asthma I |

Analysis of the Sputum Microbiome in the Severe Asthma FREE TO VIEW

Rihuang Qiu; Naijian Li; Zhaowei Yang; Mengzhang He; Xin Fen; Jing Li; Qingling Zhang; KianFan Chung; Nanshan Zhong
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State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Diseases, The First Affiliated Hospital, Guangzhou, China

Copyright 2016, American College of Chest Physicians. All Rights Reserved.

Chest. 2016;149(4_S):A14. doi:10.1016/j.chest.2016.02.016
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SESSION TYPE: Original Investigation Poster

PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM

PURPOSE: Asthma is a chronic inflammatory airway disease. There has been growing interest in the microbiome of the respiratory tract which may have a role in the chronic inflammatory process.

METHODS: We recruited 24 non-smoking patients with stable non-severe asthma, 25 severe asthmatics and 15 healthy subjects in Guangzhou. Induced sputum samples were collected for 16S ribosomal RNA analysis. The V3-V5 regions of bacterial 16S rRNA genes were amplified and sequenced by 454 pyrosequencing. Sequences were assigned to bacterial taxa by comparing them with 16s rRNA sequences in the Ribosomal Database Project.

RESULTS: Severe asthmatics had lower FEV1% predicted (57.2% vs. 86.4%, p<0.001), higher sputum eosinophil (19.5% vs. 9.0%, p<0.001) and longer duration of asthma[10.0 years vs. 7.5years, p<0.05] compared to non-severe asthmatics. There was no statistically difference in OTU numbers and diversity score between severe asthmatics, non-asthmatic and healthy subjects. At the phylum level, the difference of OTU relative abundance remained non-significant. But at family level, severe asthmatics had significant more abundance of Pseudomonadaceae (1.50%vs.0.05%, p<0.001) and Enterobacteriaceae (1.35% vs.0.01%, p<0.05) compared to non-severe asthmatics. Eosinophilic asthmatics also had more abundance of actinomycetaceae (1.35% vs.0.51%, p<0.05) compared to non-eosinophilic asthmatics. The abundance of actinomycetaceae at family level is very closely related to sputum eosinophils in asthmatics (r=0.36, p<0.01). Meanwhile, the abundance of bacteroidaceae is positive correlated with FEV1%pred (r=0.335, p<0.01).

CONCLUSIONS: Patients with severe asthma have an altered microbial composition in the respiratory tract compared with non-severe asthma. Attributes of this altered airway microbiome have been linked to clinical asthma phenotypes.

CLINICAL IMPLICATIONS: We aim to investigate the different airway microbiome between asthma phenotypes, and to confirm whether microbial colonization of the respiratory tract may have a role in the chronic inflammatory process.

DISCLOSURE: The following authors have nothing to disclose: Rihuang Qiu, Naijian Li, Zhaowei Yang, Mengzhang He, Xin Fen, Jing Li, Qingling Zhang, KianFan Chung, Nanshan Zhong

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