Disorders of the Pleura |

Evaluation of Inflammatory Mediators and Apoptosis in Pleural Mesothelial Cells and/or Neoplastic Cells Exposed to Talc Particles FREE TO VIEW

Milena Acencio, BS; Lisete Teixeira, MD; Bruna Silva; Carlos Silva; Juliana Silva; Vanessa Alvarenga; Leila Antonangelo, MD; Evaldo Marchi, MD
Author and Funding Information

Pleura Laboratory, Pulmonary Division, Heart Institute (InCor) University of Sao Paulo Medical School, Sao Paulo, Brazil

Chest. 2014;146(4_MeetingAbstracts):440A. doi:10.1378/chest.1991414
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SESSION TITLE: Pleural Disease Posters

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Wednesday, October 29, 2014 at 01:30 PM - 02:30 PM

PURPOSE: Talc pleurodesis is the most used method to prevent recurrence of malignant pleural effusion. It is discussed the role of the inflammatory response produced by pleural mesothelial cells (PMC) and neoplastic cells and the relationship of these cells with the effectiveness of pleurodesis induced by talc. The aim was to determine the role of PMC and / or neoplastic cells in the initial phase and regulation of the acute inflammatory response after instillation of talc to evaluate the production of inflammatory mediators and percentage of apoptosis.

METHODS: In this study we used cultures of human PMC extracted from transudative pleural fluid and adenocarcinoma cells (Lewis Lung Cells - LLC) divided into 5 groups: 100% of PMC, 100% of LLC, 25% of PMC + 75% of LLC, 50% of each cell line and CMP + 75% of PMC + 25% of LLC. All groups were exposed to 48 mm / talc cm2 for 48 hours and IL-6, TNF RI were measured along with the percentage of apoptosis. Statistical Analysis: One Way Anova, p< 0.05.

RESULTS: The production of both IL-6 and TNF IR was highest in the culture of 100% CMP, diminishing proportionally with the reduction in the percentage of this cell type; the culture that contained only LLC had the lowest levels of these cytokines. Conversely, the percentage of apoptosis was most evident in cultures of 100% LLC.

CONCLUSIONS: The contact of talc with the mesothelium is pivotal to the initiation of the inflammatory process with production of mediators that lead to inflammation and pleural fibrosis; besides that, talc acts selectively with higher rates of apoptosis in cancer cells.

CLINICAL IMPLICATIONS: Mesothelial cells play a key role in the initiation and regulation of the acute inflammatory response in the pleural space after talc instillation. Talc induces apoptosis in tumor cells and inhibits angiogenesis as well as promoting less aggressive action in mesothelial cells, thus contributing to better control of malignant pleural effusion.

DISCLOSURE: The following authors have nothing to disclose: Milena Acencio, Lisete Teixeira, Bruna Silva, Carlos Silva, Juliana Silva, Vanessa Alvarenga, Leila Antonangelo, Evaldo Marchi

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