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Correspondence |

Anticytokeratin (CAM5.2) Reagent Identifies Cytokeratins 7 and 8, Not Cytokeratin 18CAM5.2 Reagent Identifies CK7 and CK8 Not CK18 FREE TO VIEW

Kuang-Leei Chang, MD; Wan-Ru Chao, MD; Chih-Ping Han
Author and Funding Information

From the Department of Emergency Medicine (Dr Chang), Department of Pathology (Drs Chao and Han), Clinical Trial Center (Dr Han), and Department of Obstetrics and Gynecology (Dr Han), Chung-Shan Medical University Hospital; and Institute of Medicine (Dr Chao) and School of Medicine (Drs Chao and Han), Chung-Shan Medical University.

Correspondence to: Chih-Ping Han, MD, PhD, Department of Obstetrics and Gynecology, Chung-Shan Medical University Hospital and School of Medicine, Chung-Shan Medical University, No. 110, Sec. 1, Jianguo N Rd, Taichung, Taiwan 40201; e-mail: hanhaly@gmail.com.


Financial/nonfinancial disclosures: The authors have reported to CHEST that no potential conflicts of interest exist with any companies/organizations whose products or services may be discussed in this article.

Reproduction of this article is prohibited without written permission from the American College of Chest Physicians. See online for more details.


Chest. 2014;145(6):1441-1442. doi:10.1378/chest.14-0168
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To the Editors:

We read with great interest the contribution by Mani and Zander published in an issue of CHEST (November 2012).1 The authors presented a comprehensive and good quality review. However, one aspect of this article needs clarification, as it contains an incorrect message that we believe may misguide readers. The authors seem to have inadvertently stated that anticytokeratin (CAM5.2) identifies cytokeratins (CKs) 8 and 18.1

The source of CAM5.2 is Becton, Dickinson and Co. This antibody is derived from mouse IgG2a, clone CAM5.2. On the basis of the company’s datasheet, this clone reacts with CK7 and CK8.2,3 On the other hand, many manufacturers supplied the CK8/18 monoclonal antibody, which could be derived from the clone 5D3 (eg, Leica Microsystems GmBH, Thermo Fisher Scientific Inc, Imgenex Corp, Abcam plc, Novus Biologicals, GeneTex Inc); clones B22.1 and B23.1 (Novus Biologicals, Cell Marque Corp, GenWay Biotech Inc, Santa Cruz Biotechnology Inc, MyBiosciences.com); clone SP141 (eg, Santa Cruz Biotechnology Inc, Abcam plc, and GeneTex Inc); and clone Zym5.2 (UCD/PR-10.11; Invitrogen Corp). They reacted against CK8 and CK18.4

Sometimes CAM5.2 has been mistakenly regarded in both the clinical and anatomic pathologic communities as useful for identifying CKs 8 and 18, since previous results by Makin et al5 were attributed to breakdown products of CK8, revealing smaller molecular-weight fractions on immunoblot analysis. Becton, Dickinson and Co has revised the datasheet for CAM5.2 to indicate it has a primary reactivity with CK8 and a weaker but distinct reactivity with CK7. However, there is no reactivity with CK18 or other CKs.2,3 As a result, we clarify that CAM5.2 is not identical to antibodies CK8/18.

References

Mani H, Zander DS. Immunohistochemistry: applications to the evaluation of lung and pleural neoplasms: part 1. Chest. 2012;142(5):1316-1323.
 
Anti-cytokeratin (CAM 5.2). Research applications. Becton, Dickinson and Company website. http://www.bdbiosciences.com/external_files/is/doc/tds/Datasheets_RUO/live/web_enabled/23-1336-10.pdf. Accessed January 20, 2014.
 
Anti-cytokeratin (CAM 5.2) reagent. Becton, Dickinson and Company website. http://www.bdbiosciences.com/external_files/is/doc/tds/Package_Inserts_IVD/live/web_enabled/23-3190-02.pdf. Accessed January 20, 2014.
 
Antibodies. Biocompare website. http://www.biocompare.com/Default.aspx. Accessed January 20, 2014.
 
Makin CA, Bobrow LG, Bodmer WF. Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol. 1984;37(9):975-983.
 

Figures

Tables

References

Mani H, Zander DS. Immunohistochemistry: applications to the evaluation of lung and pleural neoplasms: part 1. Chest. 2012;142(5):1316-1323.
 
Anti-cytokeratin (CAM 5.2). Research applications. Becton, Dickinson and Company website. http://www.bdbiosciences.com/external_files/is/doc/tds/Datasheets_RUO/live/web_enabled/23-1336-10.pdf. Accessed January 20, 2014.
 
Anti-cytokeratin (CAM 5.2) reagent. Becton, Dickinson and Company website. http://www.bdbiosciences.com/external_files/is/doc/tds/Package_Inserts_IVD/live/web_enabled/23-3190-02.pdf. Accessed January 20, 2014.
 
Antibodies. Biocompare website. http://www.biocompare.com/Default.aspx. Accessed January 20, 2014.
 
Makin CA, Bobrow LG, Bodmer WF. Monoclonal antibody to cytokeratin for use in routine histopathology. J Clin Pathol. 1984;37(9):975-983.
 
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