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Lung Cancer |

The Diagnostic Value of Exhaled Breath Condensate (EBC) in Determination of Genetic Predisposition in Nonsmall Cell Lung Cancer

Mehmet Polatli, MD; Gokay Bozkurt, MD; Orhan Cildag, MD
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Adnan Menderes University Medical Faculty, Aydin, Turkey


Chest. 2013;144(4_MeetingAbstracts):656A. doi:10.1378/chest.1689807
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Abstract

SESSION TITLE: Lung Cancer Posters

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Wednesday, October 30, 2013 at 01:30 PM - 02:30 PM

PURPOSE: Some authors reported that DNA might be established in exhaled breath condensate (EBC). The changes in genetic endowment are in close relationship with some certain diseases. For this reason, it may be anticipated that which diseases are presumably occurs in groups of patients expressing specific genetic changes. However, it is not always feasible attaining appropriate samples for demonstrating such changes. The demonstration of some markers representing the inflammatory changes in lungs by EBC set somebody thinking that genetic changes may also be demonstrated in EBC. However, very few study is available on this topic. So we aimed to research if EBC is a feasible and reproducible method for sampling from the lungs by investigating the specific genetic changes for nonsmall cell lung cancer (NSCLC) and compare those changes with healthy subjects.

METHODS: Nonsmall cell lung cancer patients and voluntary subjects without having any cancer planned to include to the study. After filling the patient informed consent forms, 72 EBC materials were collected by Eco Screen-Jaeger commercial product.

RESULTS: One of the crucial step of this study was to obtain DNA from EBC and that was achieved by manual modified phenol chloroform method (by the way of increasing proteinase K amount, incubation time and potential temperatures). Although we got result by using real-time PCR method, we couldn't obtain it by using MLPA (Multiplex Ligation Dependent Probe Amplification) method which may be related with more than 80 gene probed in MLPA in contrast only few gene probed in PCR.

CONCLUSIONS: As a conclusion, DNA isolation is possible in EBC samples but the augmentation of the desired area of the gene by PCR is related with it's state is compact or not.

CLINICAL IMPLICATIONS: DNA isolation is possible in EBC samples but the augmentation of the desired area of the gene by PCR is related with it's state is compact or not.

DISCLOSURE: Mehmet Polatli: Grant monies (from sources other than industry): The project was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK) Gokay Bozkurt: Grant monies (from sources other than industry): The project was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK) Orhan Cildag: Grant monies (from sources other than industry): The project was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK)

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