Diffuse Lung Disease |

Identification of CARDS Toxin in the Bronchial Epithelial Lining Fluid After Mycoplasma pneumoniae (Mp) Inoculation: A Primate Model FREE TO VIEW

Diego Maselli, MD; Jorge Medina, MS; Peter Dube, PhD; Edward Brooks, PhD; Jacqueline Coalson, PhD; Jay Peters, MD
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University of Texas Health Science Center at San Antonio, San Antonio, TX

Chest. 2013;144(4_MeetingAbstracts):464A. doi:10.1378/chest.1704042
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SESSION TITLE: Cytokines/Cellular Interactions Posters

SESSION TYPE: Original Investigation Poster

PRESENTED ON: Wednesday, October 30, 2013 at 01:30 PM - 02:30 PM

PURPOSE: Mp produces an ADP-ribosylating and vacuolating toxin called Community Acquired Respiratory Distress Syndrome toxin (CARDS Tx) that may play a significant role in persistent inflammation in chronic pulmonary conditions, such as asthma. We determined levels of CARDS Tx in the epithelial lining fluid (ELF) of the airways of baboons after Mp exposure and examined histological changes within the lung.

METHODS: Nine baboons underwent bronchoscopic instillation of Mp 10 to the 8 ccu/10ml. ELF was collected using a microsampling brush (Olympus BC-402) on day 7. CARDS Tx was measured in the ELF using an antigen capture technique. Baboons were followed daily for clinical signs of active infection (fever, dyspnea, decreased appetite). At day 14 the baboons underwent a complete airway exam and necropsy.

RESULTS: None of the baboons developed any clinical signs of infection. Despite a completely normal endoscopic airway exam after exposure to Mp, CARDS Tx was detected in the ELF of 6/9 (67%) baboons. The CARDS Tx concentration range was 11.7-912.7 pg/ml; (mean = 328 pg/ml). All baboons had some degree of pulmonary inflammation on histology with “asthma-like” features. The group of animals in which CARDS Tx was detected had more pulmonary inflammation (10.3 vs. 7.7 points on the inflammatory panel score, p = 0.22; N.S.).

CONCLUSIONS: Significant levels of CARDS Tx can be detected 7 days after Mp exposure. CARDS Tx induces variable degrees of “asthma-like” inflammation without any signs of clinical infection.

CLINICAL IMPLICATIONS: These data suggest that subclinical infection with Mp can produce CARDS Tx and may play a role in the initiation or persistence of asthma.

DISCLOSURE: The following authors have nothing to disclose: Diego Maselli, Jorge Medina, Peter Dube, Edward Brooks, Jacqueline Coalson, Jay Peters

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