0
Original Research: Diffuse Lung Disease |

Glucose Transporter-1 Distribution in Fibrotic Lung DiseaseGlucose Transporter-1 in Lung Fibrosis: Association With [18F]-2-Fluoro-2-Deoxyglucose-PET Scan Uptake, Inflammation, and Neovascularization

Souheil El-Chemaly, MD, MPH; Daniela Malide, MD, PhD; Jianhua Yao, PhD; Steven D. Nathan, MD, FCCP; Ivan O. Rosas, MD; William A. Gahl, MD; Joel Moss, MD, PhD, FCCP; Bernadette R. Gochuico, MD
Author and Funding Information

From the Cardiovascular and Pulmonary Branch (Drs El-Chemaly, Rosas, and Moss), and the Light Microscopy Core Facility (Dr Malide), National Heart, Lung, and Blood Institute, the Radiology and Imaging Sciences, Clinical Center (Dr Yao), and the Medical Genetics Branch, National Human Genome Research Institute (Drs Gahl and Gochuico), National Institutes of Health, Bethesda, MD; the Division of Pulmonary and Critical Care Medicine (Drs El-Chemaly and Rosas), Brigham and Women’s Hospital, Boston, MA; and the Inova Fairfax Hospital (Dr Nathan), Falls Church, VA.

Correspondence to: Souheil El-Chemaly, MD, MPH, Division of Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital, 75 Francis Dr, Boston, MA 02115; e-mail: sel-chemaly@partners.org


Funding/Support: This research was supported in part by the Intramural Research Program of the National Institutes of Health (National Heart, Lung, and Blood Institute and National Human Genome Research Institute) and the National Institutes of Health [Grant 1K22HL092223, National Heart, Lung, and Blood Institute].

Reproduction of this article is prohibited without written permission from the American College of Chest Physicians. See online for more details.


Chest. 2013;143(6):1685-1691. doi:10.1378/chest.12-1359
Text Size: A A A
Published online

Background:  [18F]-2-fluoro-2-deoxyglucose (FDG)-PET scan uptake is increased in areas of fibrosis and honeycombing in patients with idiopathic pulmonary fibrosis (IPF). Glucose transporter-1 (Glut-1) is known to be the main transporter for FDG. There is a paucity of data regarding the distribution of Glut-1 and the cells responsible for FDG binding in fibrotic lung diseases.

Methods:  We applied immunofluorescence to localize Glut-1 in normal, IPF, and Hermansky-Pudlak syndrome (HPS) pulmonary fibrosis lung tissue specimens as well as an array of 19 different lung neoplasms. In addition, we investigated Glut-1 expression in inflammatory cells from BAL fluid (BALF) from healthy volunteers, subjects with IPF, and subjects with HPS pulmonary fibrosis.

Results:  In normal lung tissue, Glut-1 immunoreactivity was seen on the surface of erythrocytes. In tissue sections from fibrotic lung diseases (IPF and HPS pulmonary fibrosis), Glut-1 immunoreactivity was present on the surface of erythrocytes and inflammatory cells. BALF inflammatory cells from healthy control subjects showed no immunoreactivity; BALF cells from subjects with IPF and HPS pulmonary fibrosis showed Glut-1 immunoreactivity associated with neutrophils and alveolar macrophages.

Conclusions:  Glut-1 transporter expression in normal lung is limited to erythrocytes. In fibrotic lung, erythrocytes and inflammatory cells express Glut-1. Together, these data suggest that FDG-PET scan uptake in IPF could be explained by enhanced inflammatory and erythrocytes uptake due to neovascularization seen in IPF and not an upregulation of metabolic rate in pneumocytes. Thus, FDG-PET scan may detect inflammation and neovascularization in lung fibrosis.

Figures in this Article

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
Guidelines
Intermetatarsal neuroma.
Academy of Ambulatory Foot and Ankle Surgery | 1/16/2004
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543