Obstructive Lung Diseases |

A Disintegrin and Metalloprotease 33 Polymorphism (ADAM33) Association With COPD in Long-Term Tobacco Smokers FREE TO VIEW

Rafi Jan*, MD; Sonaullah Shah, MD; Zafar Shah, PhD; Aamir Rashid, MD
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SKIMS, Srinagar, India

Chest. 2012;142(4_MeetingAbstracts):662A. doi:10.1378/chest.1389109
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PRESENTED ON: Wednesday, October 24, 2012 at 01:30 PM - 02:30 PM

PURPOSE: 1)To evaluate thepotential relationship between SNPs ADAM33 gene and increased suse-ceptibility for development of COPD in long term toboco smokers in Kashmiri population. 2) To study whether ADAM33 gene polymorphisim have any pathogenetic role in development of COPD.

METHODS: A RANDOMISEDd case control study was conducted prospectively in departement of internal and pulmonary medicine and departement of immunology SKIMS FROM 2009 to 2011.Our study included 78 cases of COPD& they were compared with 77 age and sex matched long term smokers without COPD . Genetic analysis for three SNPs in ADAM33 gene were done by extraction and Quantification of genomic DNA.

RESULTS: High molecular weight DNA was isolated from the blood samples of COPD patients an normal healthy controls.The integrity of the DNA was checked on 1% agarose gel, DNA was subjected toPCR to ampilify the the regions of DNA containing T1,T2 and Q1 SNP.The PCR amplicons for T1 SNP, T2SNP and QQ-1 SNP were chcked on 2% agarose gel electophoresis . The Presence of diffrent snps were don by subjecting the PCR amplicons to restriction digestion,The analysis of T1 SNP , T2SNP,Q1SNP revealed GG GENOTYPE WAS HIGHER CASES OF 42.3%, 70.5% AND46.2% AS COMPARED TO CONTROLS OF 2,6%, 19,5% AND 23.4% RESPECTIVELY.

CONCLUSIONS: The predisposition of indivdual to develop COPD is related to both genetic and environmental factors. SNPS OF ADAM33 gene are significantly associted with COPD in Kashmiri POPULATION. SNPPS IN ADAM 33 NOT ONLY PREDISPOSE A IN DIVDUAL TO COPD BUT ARE ALSO ASSOCIATED WITH DISEAE SEVERITY AND RESULT IN HIGHER GOLD STAGE OF COPD.

CLINICAL IMPLICATIONS: Our Study establishes role of ADAM33 SNPS in causation of COPD

DISCLOSURE: The following authors have nothing to disclose: Rafi Jan, Sonaullah Shah, Zafar Shah, Aamir Rashid

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SKIMS, Srinagar, India




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