Diffuse Lung Disease |

Increase of Hyaluronan Levels in Bronchoalveolar Lavage (BAL) From Patients With Interstitial Lung Disease: Relationship With Proinflammatory Cytokines and Lung Function FREE TO VIEW

Glenda Ernst*, MS; Pedro Grynblat, PhD; Carolina Jancic, PhD; Auteri Santiago, MD; Fernando Galíndez, MD; Juan Carlos Moncalvo, MD; Jorge Geffner, PhD; Silvia Hajos, PhD
Author and Funding Information

María Ferrer Hospital, Buenos Aires, Argentina

Chest. 2012;142(4_MeetingAbstracts):424A. doi:10.1378/chest.1388941
Text Size: A A A
Published online


SESSION TYPE: ILD - Bench to Bedside

PRESENTED ON: Wednesday, October 24, 2012 at 02:45 PM - 04:15 PM

PURPOSE: Interstitial lung diseases (ILD) are a heterogeneous group of illnesses characterized by variable degrees of fibrosis and an imbalance of pro-inflammatory cytokines. Hyaluronan (HA) is a glycosaminoglycan which plays an important role in certain inflammatory diseases. We previously reported a significant increase in HA levels in bronchoalveolar lavage (BAL) from ILD patients compared with healthy subjects. In vitro studies have shown that HA induces the expression of pro-inflammatory genes in alveolar macrophages. In the present study we quantified HA and cytokine levels in BAL and evaluated lung function parameters in ILD patients and control subjects.

METHODS: Patient population: idiopathic pulmonary fibrosis/usual interstitial pneumonia (IPF/UIP: n=6); non-specific interstitial pneumonia (NSIP: n=4); sarcoidosis (n= 6); hypersensitivity pneumonitis (HP: n=12) and pulmonary Langerhans cell histiocytosis (Hx: n=3). Controls were individuals with healthy lungs (n=13). Cytokine and HA levels in BAL samples were determined by enzyme immunoassay. Pulmonary function tests included lung diffusing capacity for carbon monoxide (DLCO) and alveolar volume (VA).

RESULTS: We found a significant increase of HA levels in BAL from patients with NSIP (2.057,00 ± 419,90 ng/mL, p<0,01); HP (1.975,00 ± 199,30 ng/mL, p<0,001); Sarcoidosis (1.839,00 ± 47,90 ng/mL, p<0,01) and Hx (1.918,00 ± 404,20 ng/mL; p<0,05) compared with controls (623,50±48,10 ng/mL). However, there were not differences between IPF/UIP (961,90 ± 118,79 ng/mL) and the control group. When we analyzed the levels of proinflammatory cytokines (IL-1β, IL-6, TNF-α and IL-8) in BAL we observed no significant differences between ILD patients and control subjects. However, there was a correlation between IL-6 and HA levels in BAL (Spearman r = 0,57 and p<0,01). Moreover, we observed an inverse correlation between the concentrations of HA and lung functional parameters: patients with higher concentrations of HA had lower values of DLCO/VA (Spearman r = -0,28).

CONCLUSIONS: Our results show a relationship between BAL HA levels and the severity of ILD.

CLINICAL IMPLICATIONS: HA could be a marker of ILD progression.

DISCLOSURE: The following authors have nothing to disclose: Glenda Ernst, Pedro Grynblat, Carolina Jancic, Auteri Santiago, Fernando Galíndez, Juan Carlos Moncalvo, Jorge Geffner, Silvia Hajos

No Product/Research Disclosure Information

María Ferrer Hospital, Buenos Aires, Argentina




Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543