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Characterization of Eosinophils, Class II Receptor, and B Cells in Laryngeal Mucosa From an Animal Model of Sensitization and Inhalatory Challenge With Ovalbumin FREE TO VIEW

Miguel Vinuesa*, PhD; Norberto Bassan, MD; Adriel Martinez, MD; Rocio Batle, MD; Florencia Giacomozzi, MD; Valentin Torres, MD; Soledad Chaparro, MD
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Rosario School of Medicine, UNR, Rosario, Argentina

Chest. 2012;142(4_MeetingAbstracts):191A. doi:10.1378/chest.1388131
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SESSION TYPE: Cytokines/Cellular Interactions Posters

PRESENTED ON: Wednesday, October 24, 2012 at 01:30 PM - 02:30 PM

PURPOSE: In previous work we demonstrated that subcutaneous sensitization with Ovalbumin (OVA) induce generation of specific IgE antibodies and modifications of immune cells populations from bronchial mucosa in rabbit. The aim of the study is quantification of eosinophils, class II receptor and μ-chain positive cell population (B-cells) in laryngeal mucosa from OVA sensitized and challenged rabbits.

METHODS: New Zealand white rabbits were divided into three groups: G1 (n=8): normal control; G2 (n=10): subcutaneous sensitized with OVA; G3 (n=10): subcutaneous sensitized and inhalatory challenged with OVA. Four hours after challenge animals were sacrificed and obtained samples were processed for immunohistochemistry with the following monoclonal antibody: mouse anti-rabbit class II receptor and μ-chain (B-cells). Avidin-biotin-peroxidase system was used as detection kit. Cells were counted in 200 high power fields per group.

RESULTS: Data were expressed as arithmetic mean and standard deviation. Eosinophils: G1: 0.6 (SD 0.3) G2: 3.3 (SD 1.2) G3: 4.2 (SD 1.7) (G1 vs G2 and G3 p<0.001). Class II: G1: 4.0 (SD 1.3); G2: 5.1 (SD 1.8); G3: 7.8 (SD 1.7). (G1 and G2 vs G3 p<0,001). μ-chain: G1: 3.5 (SD 1.3); G2: 1.9 (SD 1.5); G3: 1.6 (SD 1.1). (G3 vs. G1 p<0.05). Specific anti-OVA-IgE levels were evaluated by positive passive cutaneous anaphylaxis test (PCA) at 160 fold dilutions.

CONCLUSIONS: An increase in number of eosinophils and Class II-positive cell is observed in sensitized and challenged animals as compared to normal control. A different pattern is expressed by μ-chain positive cells which showed a decreased after sensitization and also after challenge. We conclude that systemic sensitization with soluble antigen and inhalatory challenge induces modifications in number of immune cells populations in laryngeal mucosa infiltrate from rabbits.

CLINICAL IMPLICATIONS: Sensitization with soluble food allergen as OVA induced modifications in laryngeal mucosa infiltrate that could generate functional alterations.

DISCLOSURE: The following authors have nothing to disclose: Miguel Vinuesa, Norberto Bassan, Adriel Martinez, Rocio Batle, Florencia Giacomozzi, Valentin Torres, Soledad Chaparro

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Rosario School of Medicine, UNR, Rosario, Argentina




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