0
Original Research: Interventional Pulmonology |

Volumetric Optical Frequency Domain Imaging of Pulmonary Pathology With Precise Correlation to HistopathologyOptical Frequency Domain Imaging of Lung Pathology

Lida P. Hariri, MD, PhD; Matthew B. Applegate, BS; Mari Mino-Kenudson, MD; Eugene J. Mark, MD; Benjamin D. Medoff, MD; Andrew D. Luster, MD, PhD; Brett E. Bouma, PhD; Guillermo J. Tearney, MD, PhD; Melissa J. Suter, PhD
Author and Funding Information

From the Department of Pathology (Drs Hariri, Mino-Kenudson, Mark, and Tearney); Pulmonary and Critical Care Unit (Mr Applegate and Drs Medoff and Suter); Wellman Center for Photomedicine (Drs Hariri, Bouma, Tearney, and Suter and Mr Applegate); and Rheumatology, Allergy and Immunology Division (Dr Luster), Massachusetts General Hospital, Boston; Harvard Medical School (Drs Hariri, Mino-Kenudson, Mark, Medoff, Luster, Bouma, Tearney, and Suter), Cambridge; and Harvard-MIT Division of Health Sciences and Technology (Drs Bouma and Tearney), Cambridge, MA.

Correspondence to: Melissa J. Suter, PhD, Massachusetts General Hospital, 55 Fruit St, Warren Bldg, Room 408D, Boston, MA 02114; e-mail: msuter@partners.org


For editorial comment see page 10

Funding/Support: This work was funded in part by the National Institutes of Health [Grants R00CA134920, R37AI040618, U19AI095261, P41EB01590] and the American Lung Association [Grant RG-194681-N].

Reproduction of this article is prohibited without written permission from the American College of Chest Physicians. See online for more details.


Chest. 2013;143(1):64-74. doi:10.1378/chest.11-2797
Text Size: A A A
Published online

Background:  Lung cancer is the leading cause of cancer-related mortality. Radiology and bronchoscopy techniques do not have the necessary resolution to evaluate lung lesions on the microscopic scale, which is critical for diagnosis. Bronchial biopsy specimens can be limited by sampling error and small size. Optical frequency domain imaging (OFDI) provides volumetric views of tissue microstructure at near-histologic resolution and may be useful for evaluating pulmonary lesions to increase diagnostic accuracy. Bronchoscopic OFDI has been evaluated in vivo, but a lack of correlated histopathology has limited the ability to develop accurate image interpretation criteria.

Methods:  We performed OFDI through two approaches (airway-centered and parenchymal imaging) in 22 ex vivo lung specimens, using tissue dye to precisely correlate imaging and histology.

Results:  OFDI of normal airway allowed visualization of epithelium, lamina propria, cartilage, and alveolar attachments. Carcinomas exhibited architectural disarray, loss of normal airway and alveolar structure, and rapid light attenuation. Squamous cell carcinomas showed nested architecture. Atypical glandular formation was appreciated in adenocarcinomas, and uniform trabecular gland formation was seen in salivary gland carcinomas. Mucinous adenocarcinomas showed alveolar wall thickening with intraalveolar mucin. Interstitial fibrosis was visualized as signal-dense tissue, with an interstitial distribution in mild interstitial fibrotic disease and a diffuse subpleural pattern with cystic space formation in usual interstitial pneumonitis.

Conclusions:  To our knowledge, this study is the first demonstration of volumetric OFDI with precise correlation to histopathology in lung pathology. We anticipate that OFDI may play a role in assessing airway and parenchymal pathology, providing fresh insights into the volumetric features of pulmonary disease.

Figures in this Article

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543