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THE ROLE OF CD14 GENE EXPRESSION IN PATHOGENESIS OF MULTIPLE ORGAN DYSFUNCTION SYNDROME IN THE ELDERLY RATS FREE TO VIEW

Xingsheng Wang, MD*
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Department of Respiratory Disease, Daping Hospital, Third Military Medical Unive, Chongqing, Peoples Rep of China



Chest. 2006;130(4_MeetingAbstracts):135S. doi:10.1378/chest.130.4_MeetingAbstracts.135S-c
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Abstract

PURPOSE: To investigate the role of CD14 gene expressions in tissues in the elderly rats of multiple organ dysfunction syndrome (MODS) induced with oleic acid and lipopolysaccharide (LPS).

METHODS: Models of MODS in the elderly rats (twenty-month-old) and in the young rats (three-month-old) were established by OA (0.25ml/Kg) and LPS (3.5mg/Kg) injected (4 hours interval). Rats were randomly divided into control, 2h, 6h, and 24h groups (n=10, respectively). The pathomorphology and function of lung, heart, liver and kidney were observed. Semi-quantitative RT-PCR was used to measure the expression of CD14 mRNA in above tissues.

RESULTS: PaO2 decreased to minimum (59.3±7.41 and 67.5±8.66mmHg in Elderly Group and Young Group, •<0.01, respectively) at 2h and biochemical indexes of functional lesion of heart, liver and kidney increased to peak level at 6h. Organs injured more seriously in Elderly Group than in Young Group (P<0.05∼0.01). The expression of CD14 mRNA increased to peak level at 2h in lung tissue (1.455±0.274 vs 0.416±0.065 and 1.067±0.192 vs 0.381±0.057 in Elderly Group and Young Group, •<0.01, respectively) and at 6h in the other tissues. Which expressed most in lung tissue and more in Elderly Group than in Young Group at the same time points (P<0.01).

CONCLUSION: The quantities and function of CD14 gene are enhanced in lung tissues in the elderly rats after OA and LPS injection.

CLINICAL IMPLICATIONS: CD14 may play important roles in pathogenesis of MODS which explains the lung injured firstly and seriously.

DISCLOSURE: Xingsheng Wang, None.

Tuesday, October 24, 2006

2:30 PM - 4:00 PM


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