0
Abstract: Poster Presentations |

CHANGE OF IL-12 AND TNF-ALPHA PRODUCTIONS MEASURED BY WHOLE BLOOD CULTURE IN TUBERCULOSIS PATIENTS AFTER TREATMENT FREE TO VIEW

Young S. Kim, MD*; Jin W. Moon, MD; Chang H. Han, MD; Shin M. Kang, MD; Moo S. Park, MD; Se K. Kim, MD; Joon Chang, MD; Sung K. Kim, MD
Author and Funding Information

Yonsei Univ College of Medicine, Seoul, South Korea


Chest


Chest. 2005;128(4_MeetingAbstracts):397S-c-398S. doi:10.1378/chest.128.6.3863
Text Size: A A A
Published online

Abstract

PURPOSE:  Tuberculosis is one of the leading infectious diseases in adults, causing around 3 million deaths annually. Research on understanding the host defense and immunopathogenesis of tuberculosis is necessary because there is an urgent need for a new vaccine and adjunctive immunotherapy, particularly in patients with drug resistant Mycobacterium tuberculosis infection. Recently, many aspects of cytokine dynamics in patients with active pulmonary tuberculosis have been investigated through ex-vivo studies with specific or crude mycobacterial antigens, or M. tuberculosis.

METHODS:  We used whole blood culture method in order to evaluate IL-12 and TNF-alpha productions in active TB patients, tuberculin skin test positive and negative healthy controls. We investigated the productions of IL-12, TNF-alpha in response to nonspecific mitogens and M. tuberculosis specific antigens. We used many tuberculous antigens for stimulation. The tuberculous antigens are culture filtrate proteins (CFP), purified protein derivatives (PPD), antigen 85A M. tuberculosis proteins (Ag85A), early secretory antigen target-6 (ESAT-6), 38k Dalton antigen (38kDa), RNA polymerase β subunit B1 (rpoB1), RNA polymerase β subunits B2 (rpoB1), arabinofuranasyl lipoarabinomannan(araLAM), manosyl-capped lipoarabinomannan(manLAM), and non-specific mitogens are concanavalinA(ConA), lipopolysaccaride(LPS), phytohaemagglutinin(PHA). In addition, we monitored the changes of each cytokine production in TB patients during the course of therapeutic treatment.

RESULTS:  In our study, production of IL-12, TNF-alpha to non-mannose-capped lipoarabinomannan(araLAM) increased significantly in active tuberculosis patients compared to healthy controls, After 2 month, production of IL-12, TNF-alpha to araLAM decreased significantly throughout the therapeutic periods.

CONCLUSION:  Our study suggest that productions of IL-12, TNF-alpha plays a important role in the pathogenesis and treatment of tuberculosis.

CLINICAL IMPLICATIONS:  Whole blood culture method to IL-12, TNF-alpha may be used in the diagnosis of active pulmonary tuberculosis.

DISCLOSURE:  Young Kim, None.

12:30 PM - 2:00 PM


Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543