To investigate the tissue levels and distribution of amphotericin B in lung after i.v. administration of liposomal amphotericin B (L-AmB) in patients with lung cancer resected.
We enrolled 18 adult (>18 ys) patients with primary or secondary lung cancers. All patients were informed about the prospective study. There were 12 males and 6 females, both sexes ranging in weight from 53 to 86 kg, and in age from 28 to 65 years. L-AmB was administered by 1 hour single infusion at fixed doses of 1,5 mg/kg, and it was administered from 10 to 25 hours before the surgery. The starting and the end points of infusion were noted, the same for arterial and vein ligatures, and the end point of pulmonary tissue sample collection. Moreover, 10 ml of blood sample at the artery closure were collected. L-AmB was assayed in blood and lung tissue by an HPLC validated method of Bekersky et all (Shimadzu LC-9A pump, SPD-6A detector, CR-4A data processor, Simmetry Shield RP8 water column).
The blood levels after 10 to 16 hours from the end of infusion (n° 6) ranged from 3.4 to 2.1 mg/l-gr, while the tissue levels ranged from 0.9 to 1.59 mg/l-gr; 18 to 22 hours after blood infusion (n° 6) levels ranged from 1.8 to 0.9 mg/l-gr; 23 to 25 hours after infusion (n° 6) blood and the tissue levels ranged from 1.8 to 0.98 mg/l-gr and 1.48 to 2.38 mg/l-gr.
These data support the consistent lung tissue diffusion of L-AmB in patients with lung cancers. The L-AmB plasma concentration was gradually decreasing in all the cases.
The relationship between these data can effort the best choice of drug in possible fungal infections as for a prophylactic employment for lower dosage and for lower collateral side effects.
Cosimo Lequaglie, None.