To analyse exhaled breath condensate in subjects exposed to asbestos in the past, as experimental studies in animals have shown increased lipid peroxidation. Isoprostanes, leukotrien B4 and cysteinyl leukotriens have not yet been studied in humans exposed to asbestos.
Thirteen persons were examined, mean age 71.4 years, previous exposure to asbestos 25.5 years on average. Five subjects were diagnosed asbestosis as occupational disease, 8 had pleural hyalinoses. Control group was represented by 10 subjects, mean age 66.3 years, without occupational history of fibrogenic dusts exposure.Lung functions were measured, isoprostaglandin F2alpha; (8-isoprostane) was analyzed by HPLC/MS; leukotrien B4 by SPE, and leukotriens C4, D4, and E4 using LC/MS. Cotinine in urine was detected to confirm the smoking status. Student t-test and correlation coefficient were used for statistical comparison of the groups.
Mean level of 8-isoprostane in persons exposed previously to asbestos and controls was 60±20 and 37 ±15 pg/mL, respectively. Different values in exposed and control groups were detected (p=0.91). Mean levels of leukotriens B4, C4, D4, and E4 in exposed and controls were 42±23; 96±40; 11±5; 17±7 and 38±15; 98±47; 20±11; 23±11 pg/mL, respectively. In asbestos-exposed persons the concentration of 8-isoprostane correlated with TLCO/Hb (p=0.96) and leukotriens B4 (p=0.99), D4 (p=0.99), and E4 (p=0.99).
In preliminary results, 8-isoprostane seems to be important parameter in asbestos-exposed persons. Leukotriens levels did not differ in exposed and control groups, however in subjects with asbestos exposure leukotriens B4, D4, and E4 were positively correlated with 8-isoprostane.
The level of 8-isoprostane in the exhaled breath condensate might be a useful marker of oxidation stress also in asbestos-exposed subjects; besides that leukotriens B4, D4, and E4 seem to play a role. Acknowledgement: IGA NR/8107-3.
Daniela Pelclova, None.