To investigate G/C (Arg/Pro) polymorphism in exon 4 of the p53 tumor suppressor gene in Iranian lung cancer patients.
In this study, the frequency of the respective p53 alleles was investigated in 64 Iranian lung cancer patients and 205 ethnicity-matched healthy blood donors by an Allele-Specific Polymerase Chain Reaction method.
The frequency of Arg allele was 59% in control group compared to 49% in the lung cancer patients. The frequencies of Pro allele in control and patient groups were 41% and 51%, respectively. Accordingly, there was no significant difference in the allele and genotype frequencies between the patients and controls, as a whole. However, a highly significant increase in the Pro allele was observed in patients with small cell lung cancer compared to controls (71% vs. 41%, P = 0.009). In addition, a trend of increase in the Pro allele was observed in small cell lung cancer compared to non-small cell carcinoma of lung (71% vs. 46%, P = 0.05). No significant difference was observed in the frequencies of p53 alleles between smoker and non-smoker patients (P = 0.4).
Our data indicate that, as a whole, there is no association between p53 codon 72 polymorphism and lung cancer, however, there is an accumulation of Pro allele in the small cell lung cancer in our patients.
The presence of Pro allele of the p53 tumor suppressor gene in Iranian small cell lung cancer increases heritable susceptibility to the disease and might provide clues for lung cancer screening in our population.
Mehrnoosh Doroudchi, None.