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Abstract: Poster Presentations |

TOTAL IGE AND STIMULATED T CELL CYTOKINE PRODUCTION IN A COHORT OF ETHNICALLY DIVERSE PREGNANT WOMEN BY ASTHMA HISTORY FREE TO VIEW

Christine C. Johnson, *; M Maliarik; SL Haystad; DR Ownby; EL Peterson; G Wegienka; LK Williams; M McCabe; EM Zoratti; CL Joseph
Author and Funding Information

Henry Ford Health Systems, Detroit, MI


Chest


Chest. 2005;128(4_MeetingAbstracts):238S. doi:10.1378/chest.128.4_MeetingAbstracts.238S
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Abstract

PURPOSE:  Little is known about the relationships between total IgE as a marker for Th2 phenotype, T cell production of interferon _ (IFN-_) and IL4 as markers of Th1 and Th2 phenotypes, respectively, and IL10 as a marker of immune regulation, in a population of racially diverse females categorized by history of asthma diagnosis.

METHODS:  We analyzed blood samples taken during pregnancy from the mothers of urban and suburban African American and Caucasian children in a geographically defined Detroit area birth cohort (WHEALS). Total IgE was measured using the Pharmacia CAP low range with a detection limit of 0.1 IU/ml. IFN-_, IL4 and IL10 production by PMA stimulated lymphocytes was detected by antibody staining and flow cytometry. Results are expressed as the percentage of CD4+ cells positive for these cytokines. Wilcoxon rank sum tests were used to compare mean IgE and mean IFN-_, IL4 and IL10 percentages by history of physician diagnosed asthma and race.

RESULTS:  Blood samples from 443 women were tested; 65% were African American and 21.0% had a history of asthma. Total IgE was higher in women with asthma (155.7 vs 71.2 IU/mL, p< .001) and higher in African American women vs Caucasian women with asthma (185.8 vs 114.7 IU/mL, p< .001). IFN-_ positive cells tended to be lower in women with an asthma history (8.0% vs 9.4%, p<0.065), and lower in African American vs Caucasian asthmatics (7.4% vs 9.2%, p<0.097). There were no differences in IL4 or IL10.

CONCLUSION:  Total IgE is higher in women with a history of asthma and African American women, while IFN-_ was lower, following the Th1-Th2 paradigm. NIAID.

CLINICAL IMPLICATIONS:  Total IgE is higher in women with a history of asthma and African American women, while IFN-_ was lower, following the Th1-Th2 paradigm. NIAID.

DISCLOSURE:  Christine Johnson, None.

Wednesday, November 2, 2005

12:30 PM - 2:00 PM


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