Abstract: Poster Presentations |


Luke C. Short, PhD*; Thorsten Benter, PhD
Author and Funding Information

Bergische Universität Wuppertal, Wuppertal, Germany


Chest. 2005;128(4_MeetingAbstracts):236S. doi:10.1378/chest.128.6_suppl.582S
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PURPOSE:  An elevated concentration of nitric oxide (NO) in alveolar ventilation indicates inflammatory stress within the lung. Trace-gas analysis using mass spectrometers (MS) have been used extensively within the atmospheric community with great success. We present here the first description of time-resolved NO measurement in breath using photoionization MS, providing new capabilities for the medical investigator, such as isotopic tracing.

METHODS:  For NO measurement, we use resonance enhanced multiphoton ionization (REMPI) coupled with a time-of-flight MS with a medium pressure laser ion source. A single male subject breathes NO-free air for several minutes, and then the exhaled breath is monitored for NO.

RESULTS:  The ability of REMPI to differentiate among three different isotopomers of NO is demonstrated, and then the concentration profile of NO in exhaled breath is measured. A similar time-dependence concentration is found as observed by previous techniques.

CONCLUSION:  This study presents MPLI-MS as a new technique available for the time-resolved measurement of NO in breath. The resulting system can be made portable and brought into the medical setting using newer-generation, broad-bandwidth lasers. When measuring NO via REMPI, we found a concentration profile in agreement with that of research groups using different techniques (chemiluminescence and LMRS). Sampling the breath from a person without respiratory ailments, we found a dead-space air NO concentration of 20 ppbV and an alveolar ventilation concentration of 5 ppbV. In contrast to the chemical-based technique chemiluminescence, MPLI-MS can also selectively measure isotopically-labeled NO, as demonstrated with REMPI of 14N16O, 15N16O and 14N18O. Further, MPLI-MS can be used to measure other biologically-significant molecules, such as the aldehydes. In forthcoming studies, we will explore the application of MPLI-MS for the measurement of other biologically-significant molecules.

CLINICAL IMPLICATIONS:  (1) ppbV mixing ratios of NO can be measured on a sub-second time scale, (2) other biologically significant gas molecules can be measured on a similar time scale and (3) since the technique operates optically as well as mass-resolved, isotopomers of NO are discernable, permitting the use of isotopic tracing.

DISCLOSURE:  Luke Short, None.

Wednesday, November 2, 2005

12:30 PM - 2:00 PM




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