To evaluate the protective effect of atorvastatin(Lipitor) on the lung ischemia-reperfusion injure(LIRI) and its possible mechanism.
Single lung in site ischemia-reperfusion animal model was used. Thirty Wistar rats were randomly divided into three groups(n=10/group): a)sham operate group(SO), b)Pulmonary models of ischemia-reperfusion injury(IR) and c)atorvastatin treated group(AT). The blood flow in two latter groups were blocked up for 60 minutes, then followed by reperfusion for 120 minutes. In the AT group, the rats were fed atorvastatin (10mg/Kg) for seven days before ischemia-reperfusion. The wet-to-dry (W/D) lung weight ratio, lung permeability index(LPI), malondialdehyde contents(MDA)surperoxide dismutase(SOD) activity, myeloperoxidase(MPO) activity in the lung tissue were measured respectively. Lung tissue was observed by light microscope. Immunohistochemical technique was used to detect the inducible nitrioxide synthase(iNOS), endothelial NOS (eNOS) and surfactant protein A(SP-A) expression in lung tissues.
The levels of LPI,MDA contents, MPO activity and W/D were significantly decreased in AT group(0.0230+0.00273; 17.685±1.537; 0.0527±0.002026 and 4.65+0.11434) than in IR group(0.0301+0.00421;37.364±3.166; 0.0797±0.003902 and 769+0.1063), but the activity of SOD was significantly increased in AT group than in IR group (34.726±0.943 vs 19.728±0.817,p<0.01).The expression of SP-A and eNOS were upregulated in AT group compare with IR group (1996.584±260.081 vs 1119.609±348.256,p<0.05; 181.933±65.715 vs 69.228±16.759,p<0.05), but the expression of iNOS was significantly downregulated in AT group other than in IR group (82.294±41.150 vs 303.739±95.383,p<0.01).
The data showed that atorvastatin can significantly protect lung ischemia-reperfusion injury by upregulating the eNOS and SP-A expression and downregulating iNOS expression, and maintaining pulmonary surfactant and activity of Enos, decreasing free radicle and anti-inflammatory effect.
It is worth to further study atorvastatin protecting lung ischemia-reperfusion injury in pulmonary embolism, lung transplantation and cardio-pulmonary surgery.
Birong Dong, None.