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Kuang-Yao Yang, MD, PhD; Shih-Hwa Chiou, MD, PhD
Author and Funding Information

From the Department of Chest Medicine (Dr Yang), and the Department of Medical Research and Education (Dr Chiou), Taipei Veterans General Hospital; and the Institute of Clinical Medicine, Institute of Pharmacy (Dr Chiou), School of Medicine (Dr Yang), National Yang-Ming University.

Correspondence to: Kuang-Yao Yang, MD, PhD, Department of Chest Medicine, Taipei Veterans General Hospital, No. 201, Sec. 2, Shih-Pai Road, Taipei, 11217, Taiwan; e-mail: kyyang@vghtpe.gov.tw


Financial/nonfinancial disclosures: The authors have reported to CHEST that no potential conflicts of interest exist with any companies/organizations whose products or services may be discussed in this article.

Reproduction of this article is prohibited without written permission from the American College of Chest Physicians (http://www.chestpubs.org/site/misc/reprints.xhtml).


© 2012 American College of Chest Physicians


Chest. 2012;141(3):834-835. doi:10.1378/chest.11-3092
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To the Editor:

We thank Drs Tokalov and Bachiller for their interest in our recent article in CHEST.1 Dr Tokalov and colleagues2 have studied the role of fluorescent beads (FBs) to trace the distribution of FBs in vascular space and parenchymal organs in small animals. We have shown that IV delivery of induced pluripotent stem (iPS) cells provided a beneficial effect in attenuating the severity of endotoxin-induced acute lung injury (ALI) and resulted in a decrease in physiologic impairment. As shown by in vivo radionuclide imaging and in vitro Hoechst-labeled fluorescent staining, an increase in iPS cells in the lungs of mice with ALI was evident when compared with control mice.

We agree with Drs Tokalov and Bachiller’s recommendations that analyses of time dynamics of the distribution of iPS cells through the parenchymal organs may allow the quantification of the relative homing capabilities in the organ of interest. In our article, we have demonstrated an early-phase homing potential of iPS cells, evident by more 131I- IdUrd-labeled iPS cells accumulated over the lung area in mice with endotoxin-induced ALI than in control mice (in vivo radionuclide imaging). In addition, to explore the microscopic fate of transplanted iPS cells, we labeled iPS cells with Hoechst and located Hoechst-positive cells in lung tissue. In agreement with the findings of radionuclide imaging, after 24 h of iPS cell delivery, we found Hoechst-labeled cells were more scattered in the injured lung sections of mice with ALI when compared with lung sections of control mice. However, long-term dynamic distribution of iPS cells in different parenchymal organs may be related to the safety and efficacy of iPS cell-based therapy applied in different lung diseases and therefore requires further studies.

Notably, Drs Tokalov and Bachiller showed that FBs were initially located in the breast area of nude mice after the tail vein injection followed by the abdominal part of the body after 1 week. This elegant finding provided a novel approach to monitor the long-term dynamic distribution of FBs in vivo. In line with Drs Tokalov and Bachiller’s suggestion, this FBs-derived platform may play a role in iPS cells and other stem cell research, and further investigation of its function on migration, proliferation, differentiation, and programmed death is warranted. However, the material properties of tracer including biodegradability, biosafety, and nano-sized scale for delivery need to be examined in molecular imaging-based diagnosis and stem cell transplantation in the future.

Yang K-Y, Shih H-C, How C-K, et al. IV delivery of induced pluripotent stem cells attenuates endotoxin-induced acute lung injury in mice. Chest. 2011;1405:1243-1253. [PubMed] [CrossRef]
 
Abramyuk A, Tokalov SV. Distribution of fluorescent microspheres in vascular space and parenchymal organs of intact nude rats. Int J Radiat Biol. 2009;859:781-786. [PubMed]
 

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Yang K-Y, Shih H-C, How C-K, et al. IV delivery of induced pluripotent stem cells attenuates endotoxin-induced acute lung injury in mice. Chest. 2011;1405:1243-1253. [PubMed] [CrossRef]
 
Abramyuk A, Tokalov SV. Distribution of fluorescent microspheres in vascular space and parenchymal organs of intact nude rats. Int J Radiat Biol. 2009;859:781-786. [PubMed]
 
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