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Correspondence |

IV Delivery of Fluorescent BeadsIV Delivery of Fluorescent Beads FREE TO VIEW

Sergey V. Tokalov, PhD; Daniel Bachiller, PhD
Author and Funding Information

From the Development and Regeneration Program (Drs Tokalov and Bachiller), Fundación Caubet-Cimera; and Consejo Superior de Investigaciones Científicas (Dr Bachiller).

Correspondence to: Sergey Tokalov, PhD, Fundación Caubet-Cimera, Development and Regeneration Program, Ctra. Sóller km 12, 07110 Bunyola (Balearic Islands), Spain; e-mail: sergey.tokalov@caubet-cimera.es


Financial/nonfinancial disclosures: The authors have reported to CHEST that no potential conflicts of interest exist with any companies/organizations whose products or services may be discussed in this article.

Reproduction of this article is prohibited without written permission from the American College of Chest Physicians (http://www.chestpubs.org/site/misc/reprints.xhtml).


© 2012 American College of Chest Physicians


Chest. 2012;141(3):833-834. doi:10.1378/chest.11-2695
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To the Editor:

Cell-based therapies using induced pluripotent stem (iPS) cells have emerged as potential novel approaches for several devastating and otherwise incurable lung diseases like emphysema, pulmonary fibrosis, pulmonary hypertension, and ARDS.1 The article by Yang et al1 in a recent issue of CHEST (November 2011) evaluating the role of iPS cells in the treatment of endotoxin-induced acute lung injury revealed a possible protective effect mediated by iPS cells, which significantly diminishes the histopathologic changes of acute lung injury and the lung injury score. Moreover, it was shown that the protective effects were not replicated by control cell therapy carried with fibroblasts.

However, in our opinion, the strength of the article would be enhanced substantially by a more precise evaluation of the area of iPS cell integration into the lung and by an assessment of the iPS cell dynamic through different parenchymal organs. The latter will help to establish iPS cell lung-homing capacity.

To illustrate the importance of these points, we will describe the results of a recent experiment performed in our laboratory. A total of 1×105 fluorescent beads (FBs) (2.5 μm, λex: 630-660 nm, λem: 670-720 nm; Invitrogen) were suspended in 50 μL of phosphate-buffered saline and injected into the tail vein of Cby.CgFoxn1nu/J 1-month-old nude mice (Charles River). Location of FBs was registered by in vivo fluorescent imaging (Pearl-Impulse; LI-COR Biotechnology), at different times after the injection: 5 min, 1, 2, 3, and 4 weeks. After 4 weeks, the mice were killed according to approved methods, and their internal organs inspected for fluorescent emission. In order to localize with more precision the location of the beads, 10-μm tissue sections were analyzed by standard fluorescent microscopy (DMI 6000B; Leica Microsystems).

In agreement with our previous findings in nude rats,3 5 min after injection many of the FBs were localized in the breast area of animals (Fig 1A). However, a week after the injection almost all the FBs were present in the abdominal part of the body (Fig 1B). This image remained unaltered at 4 weeks after the injection. The analysis of autopsy material (Fig 1C, 1D) clearly shows that almost all the FBs were located in spleen and liver, being undetectable in kidney, heart, and lung.

Figure Jump LinkFigure 1. Images of male nude mice. A, 5 min. B, 1 week. C, D, after autopsy. Probe signal is displayed in red color overlaying a grayscale light image of the whole mouse (A-C), 10-μm sections of parenchymal organs (D). Scale bars correspondent to 1 cm (A-C) and 20 μm (D).Grahic Jump Location

Analyses of time dynamics of iPS distribution through the parenchymal organs might allow for the quantification of their relative homing capabilities in the organ of interest and, thereby, better clarification of their role in the different pathologic processes.

Sueblinvong V, Weiss DJ. Stem cells and cell therapy approaches in lung biology and diseases. Transl Res. 2010;1563:188-205. [PubMed] [CrossRef]
 
Yang K-Y, Shih H-C, How C-K, et al. IV delivery of induced pluripotent stem cells attenuates endotoxin-induced acute lung injury in mice. Chest. 2011;1405:1243-1253. [PubMed]
 
Abramyuk A, Tokalov SV. Distribution of fluorescent microspheres in vascular space and parenchymal organs of intact nude rats. Int J Radiat Biol. 2009;859:781-786. [PubMed]
 

Figures

Figure Jump LinkFigure 1. Images of male nude mice. A, 5 min. B, 1 week. C, D, after autopsy. Probe signal is displayed in red color overlaying a grayscale light image of the whole mouse (A-C), 10-μm sections of parenchymal organs (D). Scale bars correspondent to 1 cm (A-C) and 20 μm (D).Grahic Jump Location

Tables

References

Sueblinvong V, Weiss DJ. Stem cells and cell therapy approaches in lung biology and diseases. Transl Res. 2010;1563:188-205. [PubMed] [CrossRef]
 
Yang K-Y, Shih H-C, How C-K, et al. IV delivery of induced pluripotent stem cells attenuates endotoxin-induced acute lung injury in mice. Chest. 2011;1405:1243-1253. [PubMed]
 
Abramyuk A, Tokalov SV. Distribution of fluorescent microspheres in vascular space and parenchymal organs of intact nude rats. Int J Radiat Biol. 2009;859:781-786. [PubMed]
 
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