Other articles have demonstrated already the feasibility of such an approach for EGFR gene analyses on cytologic specimens,2,3 particularly using paraffin-embedded cell blocks. Moreover, in the recent publication on the classification of lung cancer through small biopsy specimen and cytology examination, Travis et al4 also recommended to systematically use paraffin-embedded cell blocks not only for immunocytochemistry but also for molecular analysis. In contrast, Nakajima et al1 emphasized a very important technical point: Artifacts leading to false gene mutation identification can be obtained from small DNA samples extracted from paraffin-embedded cell blocks. Specimens obtained by EBUS-TBNA often are of small size, and optimal processing is essential for their management; therefore, we agree with Nakajima et al1 that clinicians must pay attention to their treatment. Freezing and storing aliquots of the samples at −80°C in dimethyl sulfoxide according to the same procedure used for cell line preservation is easy to do. Furthermore, doing so enables optimal cell preservation for morphology and a wide range of complementary techniques, such as molecular analyses. In our institution, as already described,5 we systematically freeze a part of the cytologic specimens obtained by EBUS-TBNA at −80°C. From this frozen material, DNA extraction and sequencing usually are performed with success, whatever the specimen cellularity. These techniques are routinely performed not only on samples obtained by EBUS-TBNA, but also on other cytologic samples such as those obtained by transthoracic needle aspiration or bronchial brushings and even cerebrospinal fluid. In conclusion, we agree with Nakajima et al1 that multigene mutation analysis can be performed in EBUS-TBNA samples, promoting freezing cells rather than the cell block and, thus, ensuring optimum technical conditions.