This study was aimed to elucidated the local effects of Eotaxin, RANTES and TNF-α in the pathogenesis of atopic asthma and their relation to disease severity. Evaluate the possible effect of respiratory viral infection on studied cytokines.
Twenty nine patients with asthma were allocated to subgroups according to disease severity as severe asthma (12) and mild (17) compared with 10 control subjects. Subjects with asthma were further divided into those with respiratory virus infection (10) and those where no respiratory virus (19). RANTES, Eotaxin and TNF-α were measured in sputum. also, serum IgE was measured by ELISA. Viral culture of throat swabs from patients and pH 3 sensitivity testing was done to differentiate rhinovirus (inactivated) from enteroviruses.
The mean values of Eotaxin, RANTES, TNF-α (pg/ml), eosinophils in severe asthma are statistically higher (P<0.001) than in mild asthma and all these parameters were higher in either both groups compared to control (P<0.001). Among 29 allergic asthma patients, 10 patients were infected with respiratory viruses (34.48%), 5 patients with severe asthma and 5 with mild asthma. Seven of them were infected with Rhinovirus (24.13%). The mean values of Eotaxin, RANTES, TNF-α, eosinophils in allergic asthma with respiratory virus infection were compared to those allergic asthma without respiratory infection there were non statistically significant difference.
There was a significant role of RANTES, Eotaxin and TNF-α in the pathogenesis of atopic asthma. It may have a role in the severity of Bronchial asthma. Respiratory viral infections do not affect the sputum level of the studied cytokines.
Sputum chemokines (RANTES & Eotaxin) may be used as a new tool for grading of severity of bronchial asthma or evaluation of asthma control in the future.
Abdelbaset Saleh, No Financial Disclosure Information; No Product/Research Disclosure Information