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Abstract: Slide Presentations |

DYSREGULATION OF IFN-GAMMA AND IL-2 AND IL-8 PRODUCTION BY GAMMA DELTA T CELLS IN INDUCED SPUTUM FROM CHILDREN WITH NON-CYSTIC FIBROSIS BRONCHIECTASIS AND ITS CORRELATION WITH LUNG FUNCTION FREE TO VIEW

Nidhi Anil, PhD*; Meenu Singh, MD; Arvind Rajwanshi, MD; Harpeet Vohra, PhD
Author and Funding Information

PGIMER, Chandigarh, India


Chest


Chest. 2009;136(4_MeetingAbstracts):8S. doi:10.1378/chest.136.4_MeetingAbstracts.8S-e
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Abstract

PURPOSE:  Purpose: Non cystic fibrosis (Non-CF) bronchiectasis in childhood is an important cause of chronic suppurative lung disease in the developing countries. It is well known that T lymphocytes play a key role in the disease pathogenesis of non CF bronchiectasis. Gamma delta T cells are implicated in disease pathogenesis of several chronic disorders including asthma and tuberculosis. However their role immune dysregulation in Non-CF bronchiectasis airways remains unexplored.

METHODS:  The aim of the study was to compare the cytokine (IFN-gamma, IL-2, IL-8, IL-4 and IL-6) production by gamma delta T and alpha beta T cells upon stimulation with PMA-Io, in induced sputum of children with Non-CF bronchiectasis, (n=20; age 7–12 years) and ten healthy controls. The cytokines were estimated by flowcytometry (BD, FACScaliburTM) using CellQuest software. Furthermore association between cytokine expression and lung function was also estimated.

RESULTS:  We observed a high expression of gamma delta IFN-gamma in non CF bronchiectasis as compared to controls (34.6+0.84) Vs (10.1+0.26) p<0.01. Gamma delta IL-8 in children with non CF bronchiectasis (19.8+0.68) was significantly higher as compared to gamma delta IL-8 in controls (9.4+0.42), p<0.05. Similarly, a higher expression of gamma delta IL-2 in non CF bronchiectasis (24.6+0.78) Vs controls (12.1+0.48), p<0.05 was observed. No significant difference in IL-4, IL-6 expression between the two groups was observed. A significant negative correlation between gamma delta IL-8 & FEV1 (p<0.05) was observed.

CONCLUSION:  Gamma delta T cells contribute to the immune dysregulation in the airways of children with non CF bronchiectasis, through production of proinflammatory cytokines.

CLINICAL IMPLICATIONS:  Immune dysregulation by gamma delta T cells in the airways of children with non CF bronchiectasis could serve as a target for future therapeutic interventions.

DISCLOSURE:  Nidhi Anil, No Financial Disclosure Information; No Product/Research Disclosure Information

Monday, November 2, 2009

10:30 AM - 12:00 PM


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