Tumor necrosis factor-α (TNF-α) is known to play a role in the susceptibility to chronic obstructive pulmonary disease (COPD). There are some inconsistent data about a single nucleotide polymorphism(SNP) from different study group. There are some disease showing airway obstruction like bronchiectasis, tuberculosis destroyed lung disease. The aim of this research is to study the frequencies of TNF-α gene-308 polymorphisms in the patients showing airway obstruction in the Korean population.
A cohort of patients with airway obstruction and age matched controls were recruited from a teaching hospital in Korea. Blood sample was done and genomic DNA was extracted from peripheral blood. PCR restriction fragment length polymorphism (RFLP) was done to determine polymorphisms in the TNF-α gene-308 position.
One hundred and sixty five paients and 90 controls were studied. The frequencies of A allele in the TNF-α gene were 8.48% in patients with airway obstruction and 10% in controls. (P = 0.86 CI95% = 0.76).
There is no significant difference in the frequencies of the A allele of the TNF-α between Korean patients with airway obstruction versus controls. Therefore it may not conclude that this SNPs are independent factors in airway obstructive diseases in Korea.
Some recent studies show an association of TNF-α with COPD. However there is no data about the relationship between TNF-α and bronchiectasis or tuberculosis destroyed lung disease. Those diseases have similar clinical manifestations and decreased pulmonary functions.In this study, there was no significant difference between patients with airway obstruction and control group in the frequencies of TNF-α gene-308 position A allele. Further studies with larger numbers of COPD and other airway obstructive diseases are needed and more detailed classification of airway diseases is required.
Yongseon Cho, No Financial Disclosure Information; No Product/Research Disclosure Information