Chronic inflammation in a mainly feature of Chronic Obstructive Pulmonary Disease (COPD) and it is associated with the increased production of reactive oxygen species (ROS)so determining an increased oxidative stress in the lung.ROS are unstable elements with unpaired electrons.Macophages, Neutrophils and Eosinophils released, in patients affected by COPD, a increased amounts of ROS exceeding the reduced tissue antioxidant defences.In the Bronchoalveolar Fluid(BALF), macrophages play a main role for production of ROS in COPD.
Our aim was to verified correlation of alveolar macrophage activity with biological activity of ROS and Antioxidant Defences in BALF and in the Serum of patients affected by COPD III-IV GOLD stages.Sixteen patients affected by COPD III-IV GOLD stages were submitted to BAL, blood gas analysis, blood test samples, functional lung pletismography. In a second time we assessed ROS activity and antioxidant burden, both in serum and in macrophages supernatant fluid (D-ROM test, FRAS 4)and, finally, we studied correlation with alveolar macrophages activity. Control group was 16 healty subjects, formerly suspected affecting by COPD.
The Ratio ROS/antioxidant defences was significant raised in serum and in BALF of COPD patients; in BALF, ROS burden was strictly correlated with alveolar macrophages activity that was significantly increased. The antioxidant defences, in the serum e in BALF, were costantly overcome by ROS in COPD patients.
Alveolar Macrophages are the most important producers of ROS in the alveolar space in COPD patients III-IV GOLD stages (compared with healty people)and its directly correlated.
Air pollution and Smoke represented the most important causes of direct activation of alveolar macrophages that are directly correlated with the increased of ROS in pulmonary tissue. This represent the most important pulmonary injury and its burden depends strictly by availability of antioxidant defences.
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