Our group found that antibody production to the Mycoplasma CARDS TX (MPN372) was more robust and prolonged than the standard serologic assay(P-1 adhesin;MPN141) in a pediatric population with asthma. The purpose of this study was to evaluate the distribution of IgM and IgG to CARDS TX (MPN372) in a healthy adult population and compare that to a population of adult asthmatics.
IgM and IgG by ELISA were determined in 200 healthy control subjects for antibodies to CARDS TX. All patients with pulmonary disease or active medical illness were excluded. Mean age was 36.9 years (SD +/− 9.4). The population was ethnically diverse with 51% males/49% females. Fifty adult asthmatics with similar demographic characteristics were also analyzed. Differences were analyzed by test of equality of medians (Pearson χ2).
The mean IgG for healthy controls was 0.3559(SD:+/− 0.2281). The 95%tile and 99%tile were 0.8685 and 1.2535 respectively. The mean IgM for healthy controls was 0.2091 (SD: +/− 0.147). The 95%tile and 99%tile were 0.457 and 0.8605 respectively. Despite Mp being identified in 41.9% of our asthmatic population, the mean IgG was lower than in controls (0.2998 +/− 0.250). The median IgG was statistically higher in controls than in asthmatic subjects (Pearson χ2 = 3.727; p = 0.05).
The 95%tile and 99%tile to a highly immunogenic component of Mp (MPN 372)has been identified in a large population of healthy adults. These values should be tested against standard serologic tests in diagnosing acute Mp infection. Adult asthmatic patients appear to have lower IgG levels to Mp than a healthy adult population.
These data raise the possibility that asthmatic subjects could have a suboptimal immunologic response to Mycoplasma.
Harjinder Singh, No Financial Disclosure Information; No Product/Research Disclosure Information