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HISTOLOGICAL CHANGES IN OVALBUMIN SENSITIZED MICE PRETREATED WITH INHALED CYCLOSPORINE FREE TO VIEW

Juan F. Sanchez, MD*; Jay I. Peters, MD; Allan B. Watts; Robert O. Williams, III, PhD; Peter Dube, PhD
Author and Funding Information

University of Texas Health Science Center San Antonio, San Antonio, TX


Chest


Chest. 2008;134(4_MeetingAbstracts):s2002. doi:10.1378/chest.134.4_MeetingAbstracts.s2002
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Abstract

PURPOSE:To assess anti-inflammatory effect of inhaled cyclosporine nanoparticles in the histology of a rodent model of asthma.

METHODS:A total of 16 mice were injected intraperitoneally with 10% ovalbumin on days 1 and 14 and on day 28 they were allocated to 2 treatment groups: 8 mice received 72 mg of inhaled cyclosporine nanoparticles diluted in lecithin and 8 received inhaled normal saline (placebo). These were compared to 5 controls. Following the treatment, all groups except the controls, were exposed to 10% nebulized ovalbumin followed by lung harvesting 24 hours later. Airway inflammation was graded for severity in a blinded fashion with an inflammation score of 0 to 3 with 0-normal, 1-mild, 2-moderate and 3-severe inflammation.

RESULTS:The mean inflammation scores were: inhaled normal saline (placebo) 2.25±0.25, inhaled cyclosporine nanoparticles 0.62±0.21 and control group 0. Cyclosporine had significantly lower inflammatory scores compared to placebo (0.62 and 1.81 respectively versus 0; p< 0.0001, 95% confidence interval).

CONCLUSION:This pilot study suggests that specific targeting of T-cells with inhaled anti-inflammatory agents such as cyclosporine control the inflammatory component of bronchial asthma. Future studies are needed in order to test efficacy and safety of this formulation as anti-inflammatory agents in asthma.

CLINICAL IMPLICATIONS:Inhaled cyclosporine can be used as an additional agents to contol asthma.

DISCLOSURE:Juan Sanchez, No Financial Disclosure Information; No Product/Research Disclosure Information

Monday, October 27, 2008

10:30 AM - 12:00 PM


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