PURPOSE: To investigate the molecular mechanism on genomic level of using Fei-Yan-Ning Granule in treating transplanted human lung carcinoma in nude mice.
METHODS: Nude mice with transplanted human lung carcinoma H 460 tumor were randomly divided into three groups: the model group (group A), chemotherapy treated group (group B) and Feiyanning Granule treated group (group C). The total RNA were isolated from carcinoma tissue of groups, the changes of genes expression profile of tumor tissues were evaluated by cDNA microarray technology.
RESULTS: As compared with the control group, the chemotherapy treated group showed 2187 genes changes. The 997 genes were up-regulated and 1190 genes were down-regulated. The Feiyanning Granule treated group showed 2093 genes changes in their gene expression profiles. The 968 were up-regulated and 1125 gene were down-regulated, involving multiple scopes as cell cycle, signal tranduction, transcription factor, DNA repair, RNA processing and protein synthesis, energy metabolism, cell receptors as well.
CONCLUSION: The antitumor effect of Feiyanning granule is multi-targeted, which elucidates part of the pharmacologic mechanism of Feiyanning granule in molecular level.
CLINICAL IMPLICATIONS: Both clinical and laboratorial studies showed that Feiyanning granule had the effects on lung cancer.
DISCLOSURE: Juyong Wang, No Financial Disclosure Information; No Product/Research Disclosure Information