0
Abstract: Poster Presentations |

FLOW CYTOMETRY OF BAL CYTOKINES IN PATIENTS WITH IDIOPATHIC PULMONARY FIBROSIS AND ORGANIZING PNEUMONIA FREE TO VIEW

Ilias Papanikolaou, MD*; Vasileios Skouras, MD; Apostolos Voulgaridis, MD; Marianna Tsaramirsi, MD; Dimitrios Mermigkis, PhD; Konstantina Tsakanika, MD; Napoleon Karagiannidis, PhD; Vlasis Polychronopoulos, PhD
Author and Funding Information

Sismanoglio General Hospital, Athens, Greece


Chest


Chest. 2008;134(4_MeetingAbstracts):p114002. doi:10.1378/chest.134.4_MeetingAbstracts.p114002
Text Size: A A A
Published online

Abstract

PURPOSE:Th2 and Th1 cytokines are involved in the pathogenesis of Idiopathic Pulmonary Fibrosis (IPF) and Organizing Pneumonia (OP), respectively. The aim of this study was to evaluate by flow cytometry, rather than Enzyme-Linked ImmunoSorbent Assay (ELISA) routinely performed, the expression of these cytokines in Bronchoalveolar Lavage (BAL) of these patients and their correlation with clinical markers.

METHODS:The study prospectively evaluated BAL titers of IL-1β, IL-6, IL-8, IL-10, IL-12 and TNF-α in seventeen patients with IPF (group A, n=11) and OP (group B, n=6). Control group consisted of five healthy volunteers. Diagnosis of IPF was made by lung biopsy or ATS/ERS criteria. Diagnosis of Organizing Pneumonia was based on lung biopsy. BAL extracellular cytokines were measured by a Cytometric Bead Array assay (CBA) by Beckton-Dickinson. Results were compared between groups A, B and controls and correlated with BAL‘s absolute neutrophils and lymphocytes, age and the Composite Physiologic Index (CPI).

RESULTS:IL-6, IL-8, IL-1β and IL-10 were found significantly elevated in group A compared to controls (p <0,05). TNF-α and IL-12 were significantly elevated in group B compared to controls (p <0,05). Group A exhibited statistically marked elevation of IL-6, IL-8, IL-1β and IL-10 compared to group B (p <0,05). In group B, IL-12 was also significantly elevated compared to group A (p <0,05). Absolute BAL neutrophil counts in group A correlated positively with IL-10 (p<0,05), while absolute BAL lymphocytes in group B were inversely correlated with IL-1β (p<0,05). Correlation with age or CPI (in group A) was not significant.

CONCLUSION:Results are similar to studies using ELISA. Activation inducing cytokines and IL-10 in IPF patients promote anti-Th1 activity and neutrophils accumulation. Balance of anti-fibrotic (IL-12) to anti-inflammatory (IL-10) cytokines is down-regulated. In OP patients, IL-12 and Th1 pathway prevail.

CLINICAL IMPLICATIONS:Apart from Cluster Designation (CD) assignment, BAL flow cytometry may provide through soluble cytokines clinical clues to diagnosis. Balance between protective, acute phase and fibrotic cytokines may help to explain different clinical manifestations.

DISCLOSURE:Ilias Papanikolaou, No Financial Disclosure Information; No Product/Research Disclosure Information

Wednesday, October 29, 2008

1:00 PM - 2:15 PM


Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543