PURPOSE:Initial injury from smoke inhalation is mainly to the trachea and bronchi and is characterized by mucosal hyperemia and increased microvascular permeability. Vascular endothelial growth factor (VEGF) increases microvascular permeability. Recently, angiopoietin-1 and angiopoietin-2 have been suggested to play a coordinated role in regulating microvascular permeability stimulated by VEGF. This study was designed to examine the roles of angiopoietin-1 and angiopoietin-2 in smoke inhalation injury.
METHODS:We assessed victims (n = 6) of smoke inhalation. We studied the clinical symptoms, laboratory data, and chest X-ray. We also performed the bronchoscopy 5 days, 12 days after they were exposed to smoke inhalation. Serum and bronchoalveolar lavage fluid (BALF) were collected and VEGF, angiopoietin-1 and angiopoietin-2 levels were measured by ELISA.
RESULTS:Significantly greater concentrations of VEGF (mean, 156.5 pg/mL vs 51.7 pg/mL; p < 0.05), angiopoietin-1 (mean, 2459.1 pg/mL vs 1989.6 pg/mL; p < 0.05) and angiopoietin-2 (mean, 1877.5 pg/mL vs 530.9 pg/mL; p < 0.05) were seen in the serum (n = 6) compared with control subjects (n = 6). In the analysis of BALF, smoke inhalation showed significantly greater concentrations of angiopoietin-1 (mean, 2580.4 pg/mL vs 144.8 pg/mL; p < 0.05) and angiopoietin-2 (mean, 18845.0 pg/mL vs 1471.3 pg/mL; p < 0.05) compared with control subjects. However, the increase was not significant in VEGF level of BALF (mean, 211.2 pg/mL vs 204.3 pg/mL; p > 0.05).
CONCLUSION:In smoke inhalation injury, the levels of VEGF, Angiopoietin-1 and angiopoietin-2 were increased in serum and BALF.
CLINICAL IMPLICATIONS:These data suggested that the VEGF, angiopoietin-1 and angiopoietin-2 plays a critical role in smoke-induced lung injury and offer an attractive therapeutic approach for this injury.
DISCLOSURE:Yang Deok Lee, No Financial Disclosure Information; No Product/Research Disclosure Information