PURPOSE:Recent studies have suggested that chronic and recurrent infections may be linked to the development of coronary artery disease (CAD). This study investigated the relationship between Chlamydia Pneumoniae (C Pneumoniae) and CAD via detection of C Pneumoniae DNA and some inflammatory markers from Coronary Sinus during Coronary angiogram and angioplasty.
METHODS:Sixty two prospective consecutive patients referred for coronary angiography ± intervention were enrolled into the study (54 men, 8 women) mean age 56 ±11(range: 18–82) years. Indications for angiography were; chest pain/angina, unstable angina and acute coronary syndrome ( 23, 24, and 15 patients respectively). The study group consisted of 52 patients with CAD needed angioplasty and stenting and 10 patients with normal coronary arteries or minimal disease. Coronary sinus blood samples were drawn immediately before coronary angiography or before intervention and within 3 to 5 minutes after stenting. We used sodium/perchlorate/chloroform DNA extraction technique and HL-1/HR-1 primers amplifying a genomic 438-bp C. pneumoniae target sequence to detect C. pneumonia.Coronary sinus blood samples were also tested for some inflammatory markers like: hs-CRP, BNP, ACE, myoglobin, troponin T, lactate, and beta hydroxyl butyrate.
RESULTS:Thirty four patients were diabetic (55%), 39 hypertensive (63%), 32 dyslipidemic (52%), 24 smoker (39%) and 22 had left ventricular dysfunction (35%). C. Pneumoniae DNA test was negative in all patients (100%) with or without CAD as well as after coronary angioplasty and stenting. Although there was a significant difference in the beta hydroxyl butyrate level before and after coronary intervention (1.84 ± 1.46 and 2.29 ± 1.57 mg/dl, p= 0.005), all other tested inflammatory and biochemical markers were similar before and after intervention. (p>0.05). (Table).
CONCLUSION:We conclude from this pilot study that C. Pneumoniae is not assigned as coronary artery disease risk factors for our geographical region. there was no significant change in most of biochemical and inflammatory markers post coronary intervention(p>0.05) except for beta hydroxy butyrate.
CLINICAL IMPLICATIONS:C. Pneumoniae is not assigned as coronary artery disease risk factors for our geographical region.
DISCLOSURE:Walid Hassan, No Financial Disclosure Information; No Product/Research Disclosure Information