PURPOSE: To explore the characteristics of SARS Coronovirus-induced lung injury by comparing the protein expression of CD34,VEGF and AQP-1 in SARS lungs with those in non-SARS lung injury.
METHODS: Lung autopsy specimens, from 7 diagnosed SARS cases and 6 classic ARDS cases, were examined by routine Hematoxylin and Eosin (HE) and immunohistochemistry stain of CD34, VEGF and AQP-1. Normal lung from 4 patients with lung cancer was as the control. The staining density was acquired and compared between SARS and non SARS group.
RESULTS: The blood vessels in SARS lungs outlined the alveolus clearly, CD34 mean optical density(M.O.D) was 0.2843 ± 0.1033,which was significantly higher than that of non-SARS lungs(0.1297 ± 0.0073,P = 0.0074). The M.O.D of VEGF of the two groups had no statistical difference. The M.O.D of AQP-1 in SARS and non-SARS lung was significantly decreased but without significant difference between the two groups. M.O.D for SARS was 0.09710±0.02413, and 0.09830±0.01780 for classic ARDS(p=0.922).
CONCLUSION: The injury to pulmonary endothelial cells in SARS was not as serious as in classic ARDS. The expression of VEGF and AQP-1 was very similar to that of classic ARDS. The study might be contributable to the understanding of lung injury caused by SARS.
CLINICAL IMPLICATIONS: The study revealed some features of SARS virus induced lung injury.These will perhaps explain the clinical features and responsiveness to treatments of SARS.
DISCLOSURE: Guangfa Wang, None.