PURPOSE: Cysteinyl leukotrienes (Cys-LT) are known to cause local and systemic inflammatory responses. Elevated levels of Cys-LT have been investigated in graft dysfunction and rejection after solid organ transplantation. These effects in lung transplant recipients are not well defined. We hypothesized that Cys-LT levels in bronchoalveolar lavage (BAL) fluid would be elevated in lung transplant recipients with bronchiolitis obliterans syndrome (BOS), a clinical correlate of chronic airway rejection.
METHODS: Patients with and without BOS were identified by retrospective chart review using spirometry data. Cys-LT levels were measured in previously collected and stored BAL samples by ELISA. Paired student t-test was used to investigate the differences between the two groups.
RESULTS: Nine specimens were collected from patients with a diagnosis of BOS (Stage 1 or higher) and 23 specimens were collected from patients without any evidence of rejection, infection or BOS. Mean (±SEM) Cys-LT level in the BOS group was 8128 (±4922) pg/ml compared to 2708 (±978) pg/ml in the non-BOS group (p=0.14).
CONCLUSION: Patients with BOS had a higher mean Cys-LT level in the BAL fluid compared to patients without BOS. There was no statistically significant difference between the levels of Cys-LT in patients with and without BOS.
CLINICAL IMPLICATIONS: Further studies are needed to investigate the role of arachidonic acid metabolites in the development of graft dysfunction after lung transplantation.
DISCLOSURE: Binal Kancherla, No Financial Disclosure Information; No Product/Research Disclosure Information