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Abstract: Poster Presentations |

COMPARISON BETWEEN TUMOR NECROSIS FACTOR-A IN INDUCED SPUTUM AND TNF-A PROMOTER GENE POLYMORPHISM IN KOREAN COPD FREE TO VIEW

Yu Il Kim, MD; Sung Chul Lim, MD*; Kyu Sik Kim, MD; Young Chul Kim, MD; In Jae Oh, MD; Jin Young Ju, MD; Gye Jung Cho, MD; Dong Yeol Chae, MD; Jung Hwan Lim, MD
Author and Funding Information

Chonnam National University Hospital, Gwangju, South Korea


Chest


Chest. 2007;132(4_MeetingAbstracts):522. doi:10.1378/chest.132.4_MeetingAbstracts.522
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Abstract

PURPOSE: A polymorphism of TNF-α has been reported, but inconsistent results may arise from different populations of COPD. This research was conducted to study the TNF-α polymorphisms and to investigate the association between genetic polymorphisms and levels of TNF-α in the sputum.

METHODS: 25 COPD patients and 13 controls were recruited. Genomic DNA was used as a template for amplification by PCR to determine the TNF-α polymorphism. The products were investigated by auto-sequencing analysis. The level of TNF-α in induced sputum was measured by ELISA.

RESULTS: The levels of TNF-α were significantly increased in sputum of COPD patients(53.410.7pg/ Image not available.) as compared with control subjects(19.711.9pg/Image not available.). The level of TNF-α in induced sputum was inversely correlated with FEV1 (r= −0.42, p=0.008). The frequencies of GG genotype in the TNF-α gene promotor region were 88.0%(22/25) in the COPD and 84.6%(11/13) in the controls. The frequencies of GA genotype in the TNF- gene promotor region were 12.0%(3/25) in the COPD and 15.4%(2/13) in the controls. There was no association between TNF- polymorphism and TNF- levels of sputum.

CONCLUSION: The level of the TNF-α was markedly increased and inversely correlated with airflow obstruction. Although it has been speculated that TNF-α might have a causal relationship with COPD,TNF-α gene promoter polymorphism does not seem to play a major role as genetic risk factor in Korean COPD.

CLINICAL IMPLICATIONS: However, we cannot exclude that other mutations in the TNF gene complex may play a role. Further study in association with mutations in other genes will be needed.

DISCLOSURE: Sung Chul Lim, No Financial Disclosure Information; No Product/Research Disclosure Information

Wednesday, October 24, 2007

12:30 PM - 2:00 PM


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