PURPOSE: To investigate the association between the β2-adrenergic receptor gene polymorphisms and chronic heart failure (CHF) in Southern Chinese Han population.
METHODS: Genomic DNA was extracted from blood leukocytes from patients with CHF (n=92) and from healthy controls (n=80). The part of the β2-adrenergic receptor gene was amplified by polymerase chain reaction (PCR) and analysed by automated sequencing.
RESULTS: There were four single nucleotide polymorphisms (SNPs) of β2-adrenergic receptor gene: 16Arg/Gly, 27Gln/Glu, 87LeuA/G and 178ArgA/C. Mono-locus analysis showed the genotype of 27 Gln/Glu were all C/C in controls, while there were seven G/C in CHF patients, no G/G were found. The difference of genotype and allele were significant between two groups (P<0.01). There was no significant difference between two groups of other SNPs (P>0.05). From the clinical data analysis of CHF patients, LVEF was worse in G/C genotype group than in C/C group (39% versus 51%, respectively; P=0.004), the proportion of NYHA 4 was more in G/C genotype group than in C/C group (57.1% versus 18.5%, respectively; P=0.04). The haplotype analysis showed the frequency of 16G-27C-87G in CHF patients was significantly decreased as compared with controls (0.095 versus 0.191, respectively; OR, 0.445 [95%CI, 0.277 to 0.715]; P<0.01).
CONCLUSION: The 27Gln/Glu heterozygous is probably a susceptible genotype of CHF, which is related to the severity of CHF and left ventricular function. The 16G-27C-87G is probably a protecting haplotype of CHF.
CLINICAL IMPLICATIONS: The β2-adrenergic receptor gene polymorphisms was associated with CHF in Southern Chinese Han population.
DISCLOSURE: Xumin Hou, No Financial Disclosure Information; No Product/Research Disclosure Information