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Abstract: Poster Presentations |

SUPPRESSION OF RIGHT VENTRICULAR HYPERTROPHY AFTER EXTENSIVE PULMONARY RESECTION IN RATS BY GRANULOCYTE COLONY-STIMULATING FACTOR FREE TO VIEW

Shinsuke Matsumoto, MD*; Hisashi Iwata, MD; Koyo Shirahashi, MD; Tsutomu Marui, MD; Yoshimasa Mizuno, MD; Hirofumi Takemura, MD
Author and Funding Information

Gifu University, Gifu-City, Japan


Chest


Chest. 2009;136(4_MeetingAbstracts):136S. doi:10.1378/chest.136.4_MeetingAbstracts.136S-a
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Abstract

PURPOSE:  The objective of the present study was to investigate the effects of granulocyte colony-stimulating factor (G-CSF) on right ventricular hypertrophy following extensive pulmonary resection in rats.

METHODS:  Adult rats were divided into four groups: 1) Group S (right thoracotomy only); 2) Group L (right three lobectomy); 3) Group LG10 (Group L + G-CSF (10 μg/kg/day)); and 4) Group LG100 (Group L + G-CSF (100 αg/kg/day)). At postoperative day 21, weight ratio of the right ventricular to the left ventricle plus septum (RV/LV+S, indicator of right ventricular hypertrophy) were measured, and a histopathological study was conducted to determine percentage wall thickness of peripheral pulmonary arteries and proliferating cell nuclear antigen labeling index (indicator of oxidative DNA damage) of right ventricles.

RESULTS:  Mean RV/LV+S for Group S was 0.27 ± 0.02, significantly smaller than that for the lobectomy groups (Group L, LG10, LG100; 0.47 ± 0.05, 0.35 ± 0.02, 0.38 ± 0.05). G-CSF significantly suppressed right ventricular hypertrophy. Mean medial wall thickness of peripheral pulmonary arteries for Group S was 13.6 ± 4.9%, significantly smaller than that for Group L (22.9 ± 9.6%). Compared to Group L, G-CSF reduced medial wall thickness (LG10, 17.6 ± 9.5%; LG100, 18.0 ±11.2%). Incidence of proliferating cell nuclear antigen positive nuclei for Group S was 1.07 ± 0.49%, significantly smaller than that for Group L (13.77 ± 5.87%). G-CSF significantly reduced the incidence of proliferating cell nuclear antigen positive nuclei (LG10, 4.04 ± 2.14%; LG100, 3.18 ± 1.66%).

CONCLUSION:  G-CSF administration not only reduce medial wall thickness of peripheral pulmonary arteries, but also directly protect cardiomyocytes of the right ventricle, thus suppressing right ventricular hypertrophy.

CLINICAL IMPLICATIONS:  These results suggest that low-dose G-CSF administration can prevent right heart failure following extensive pulmonary resection.

DISCLOSURE:  Shinsuke Matsumoto, No Financial Disclosure Information; No Product/Research Disclosure Information

Wednesday, November 4, 2009

12:45 PM - 2:00 PM


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