The objective of the present study was to investigate the effects of granulocyte colony-stimulating factor (G-CSF) on right ventricular hypertrophy following extensive pulmonary resection in rats.
Adult rats were divided into four groups: 1) Group S (right thoracotomy only); 2) Group L (right three lobectomy); 3) Group LG10 (Group L + G-CSF (10 μg/kg/day)); and 4) Group LG100 (Group L + G-CSF (100 αg/kg/day)). At postoperative day 21, weight ratio of the right ventricular to the left ventricle plus septum (RV/LV+S, indicator of right ventricular hypertrophy) were measured, and a histopathological study was conducted to determine percentage wall thickness of peripheral pulmonary arteries and proliferating cell nuclear antigen labeling index (indicator of oxidative DNA damage) of right ventricles.
Mean RV/LV+S for Group S was 0.27 ± 0.02, significantly smaller than that for the lobectomy groups (Group L, LG10, LG100; 0.47 ± 0.05, 0.35 ± 0.02, 0.38 ± 0.05). G-CSF significantly suppressed right ventricular hypertrophy. Mean medial wall thickness of peripheral pulmonary arteries for Group S was 13.6 ± 4.9%, significantly smaller than that for Group L (22.9 ± 9.6%). Compared to Group L, G-CSF reduced medial wall thickness (LG10, 17.6 ± 9.5%; LG100, 18.0 ±11.2%). Incidence of proliferating cell nuclear antigen positive nuclei for Group S was 1.07 ± 0.49%, significantly smaller than that for Group L (13.77 ± 5.87%). G-CSF significantly reduced the incidence of proliferating cell nuclear antigen positive nuclei (LG10, 4.04 ± 2.14%; LG100, 3.18 ± 1.66%).
G-CSF administration not only reduce medial wall thickness of peripheral pulmonary arteries, but also directly protect cardiomyocytes of the right ventricle, thus suppressing right ventricular hypertrophy.
These results suggest that low-dose G-CSF administration can prevent right heart failure following extensive pulmonary resection.
Shinsuke Matsumoto, No Financial Disclosure Information; No Product/Research Disclosure Information