To explore the inhibitory effect of okam extract J201 on bleomycin-induced pulmonary fibrosis and its underlying mechanism of action.
Inhibition of proliferation and collagen synthesis of human lung fibroblast by okam extract J201 and its effect on PDGF were measured by MTT assay and Sircol Soluble Collagen Assay in vitro. Using bleomycin-induced pulmonary fibrosis animal model, its effects on mouse lung index, concentration of serum IL-4, INF-r were measured with ELISA. The concentrations of MDA and hydroxyproline in supernant of lung tissue were measured and microscopic pathology of lung was evaluated at the same time.
Okam extract J201 significantly inhibited the proliferation and collagen synthesis of lung fibroblast induced by PDGF with its peak inhibitory rate at concentration above 50ug/ml. Serum IL-4 was significantly lower and INF-r higher in okam group than those in controls. The contents of MDA and hydroxyproline in mouse lung supernant were significantly lower in okam group than in controls. Mouse lung index as measured by lung weight divided by body weight were significantly lower in okam group than that in control. Light microscopic examination revealed less inflammatory cell infiltration and fibrosis of the lung.
Okam extract J201 inhibits bleomycin-induced pulmonary fibrosis in mouse possibly by inhibition on fibroblast proliferation and Th2 cell cytokines.
Okam extract J201 may be used for the treatment of pulmonary fibrosis.
H. Jiang, None.