Mechanical Ventilation MV and orotracheal intubation are consider the major risk factor to develop Ventilator associated Pneumonia VAP. Our objective is to compare H and bacteriological B findings on healthy lungs during 6 to 72h of MV.
MV protocol was conducted in HP during 6, 12, 18, 24, 48 and 72 h. In vivo/post mortem respiratory samples were obtained to H and B evaluation.
All animal under MV< 24 h, showed different grade of tissue inflammation and neutrophiles aggregation, but pneumonia was absent. B growth from lung cultures was considerated colonization. HP ventilated > 24h had a different grade of HB change suggested of pneumonia from severe acute inflammatory infiltrate, localized-confluent consolidation zones, and on basal zones an abcessed areas was observed. H and B correlation of pneumonia was identified in cultures with moderate to heavy concentration of E. Coli, Streptococcus sp and Staphylococcus coag. Negative.
The Morelia Experience data show that develop of VAP is strongly associated to the length of MV (late phase >24h vs early phase <24h) Future clinical research in animals are mandatory to define this information in order to evaluate the possible benefict in humans.
To consider the possibility to decrease the exposure period of MV and/or use others alternatives as non invasive MV or rescue extubation when possible.
E.A. Maldonado-Ortiz, None.