The aim of this work was to verify i) whether GER may worsen asthma by exacerbating the pre-existing airways inflammation and oxidative stress and ii) investigate the validity of breath condensate analysis in the study of the airways of subjects with GER.
We enrolled fourteen patients affected by mild asthma associated with GER (49±13 yr), nine with mild persistent asthma (39±13 yr), eight with GER (35±11 yr) and seventeen healthy subjects (33±9 yr). Sputum cells count and levels of IL-4, IL-6 and 8-isoprostane in breath condensate and supernatant were measured.
A high airways neutrophilic inflammation has been observed in the airways of subjects with GER and GER and asthma, as assessed by the elevated concentrations of IL-6 observed in their breath condensate and supernatant of sputum and by cell’s sputum analysis. A concomitant increase in 8-isoprostane, as a marker of oxidative stress, has been found in the airways of these subjects. We conclude that GER is characterized by an oxidative stress that seem to worsen that already existing in asthma and by an airways neutrophilic inflammation.
The measurement of inflammatory and oxidant markers in the breath condensate of subjects with GER mirrors that observed in the induced sputum.
We believe that the use of breath condensate may be very useful in the study and monitoring of respiratory damage due to GER.
G.E. Carpagnano, None.