To evaluate the viability and function of patches of autologous myometrium to infarcted myocardium of rabbits.METHOD: Seven rabbits weighing between 5-6 pounds were anesthetized and intubated. The heart was exposed via midline sternotomy. Myocardial infarction was induced by ligating left anterior descending coronary artery, which was verified by ST segment changes. Then via laparotomy incision, a small segment of uterus was removed and applied to infarcted myometrium. This was reinforced by greater omentum.RESULT: PET scanning of 3 rabbits revealed viable myometrium with normal perfusion. Ventriculogram on 4 rabbits revealed slight dyskenesae of the posterior wall with ejective fraction of 42%. Histology revealed viable myometrium which is firmly adhered to the infarcted left ventricule demonstrating excessive angiogenesis and estrogen, progesterone receptors.
Experimental myometrial cell patch Cardiomyoplasty to infarcted myocardium revealed viable myometrium which is well adhered to infarcted myocardium. The viability was also further demonstrated by positive stain for smooth muscle actin, estrogen or progesterone receptors. Transplanted myometrium synchronous contraction and satisfactory ejection fraction.
The possibility of this unusual differentiation of myometrial cell to cardiomyocyte phenotype may have a significant impact on the use of human myometrium as tissue or cell therapy for cardiomyopathic patients.
S.A. Taheri, None.