Abstract: Poster Presentations |

No Effect of Allergen Exposure on Ciliary Beat Frequency of Allergic Tissue in vitro: Dose Response Study FREE TO VIEW

Thorsten Stein, MD; Mohamed Y. Abdel-Aziz, MD; Inge Wissen-Siegert, MD; Hans L. Hahn, MD
Author and Funding Information

Deutsche Klinik für Diagnostik, Wiesbaden, Germany


Chest. 2003;124(4_MeetingAbstracts):140S. doi:10.1378/chest.124.6.2324
Text Size: A A A
Published online


PURPOSE:  To assess effects on ciliary beat frequency (CBF) of two doses of allergen (low and high concentration x time product).

METHODS:  We examined nasal cilia from 29 patients with allergic rhinitis (14M, 15F, age 36±12) in vitro following turbinate surgery. Allergic rhinitis was confirmed by allergy testing and clinical history. The two doses of allergen used were 500 biological units/ml (standard intracutaneous test strength) for 15 minutes (group 1) and 2500 biological units/ml for 30 minutes (group 2). Tissues were suspended in medium M199 in Earle’s balanced salt solution, equilibrated with carbogen. Tissues were washed to remove blood and drugs. From each specimen two biopsies were obtained and suspended in allergen or control solution in randomized sequence. Tissues were studied in the shallow trough of a microscopy slide closed with a cover glass, and kept at 37° C throughout. We employed transmitted light microscopy (magnification 500 x, Zeiss) and photometry (Zeiss) to assess CBF. The photometer signal was digitized (Sound Technology ST191 acquisition module) and subjected to online Fourier analysis (SpectraPro FFT analysis system). CBF was assessed continuously and logged every 15 seconds.

RESULTS:  Mean CBF was 11.2 Hz immediately after suspension in control solution and was 11.2 Hz in allergen (p = 0.92, low dose group). Values were 11.6 vs. 11.9 Hz in the high dose group (control vs. allergen, p = 0.44). During the subsequent 15 or 30 minutes CBF did not change significantly, being 11.6 vs. 10.5 Hz (control vs. allergen, p = 0.36, min. 15) in group 1 and 12.1 vs. 11.6 Hz (control vs. allergen, p = 0.6, min. 30) in group 2. Thus, neither dose of allergen changed CBF significantly compared to control.CONCLUSIONS: Allergen does not change CBF of allergic tissue in vitro at concentration x time products of 500 x 15 and 2500 x 30 (biological units/ml x minutes).

CLINICAL IMPLICATIONS:  Impaired ciliary beating is not a likely cause of mucus problems in respiratory tract allergy.

DISCLOSURE:  T. Stein, None.

Wednesday, October 29, 2003

12:30 PM - 2:00 PM




Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543