Abstract: Slide Presentations |

Contribution of IL-5 and IL-13 in Pneumothorax-associated Pleural Eosinophilia FREE TO VIEW

Ioannis T. Kalomenidis, MD; Yubiao Guo, MD; R Stokes Peebles, MD; Michael Hawthorne, MD; Kenneth B. Parkes, Student; Kirk Lane, PhD; Gregory Hanley, DVM, PhD; Richard W. Light, MD, FCCP
Author and Funding Information

Department of Pulmonary Medicine, St Thomas Hospital, Department of Allergy, Pulmonary and Critical Care Medicine and Division of Animal Care, Vanderbilt University, Nashville, TN


Chest. 2003;124(4_MeetingAbstracts):81S. doi:10.1378/chest.124.4_MeetingAbstracts.81S
Text Size: A A A
Published online


PURPOSE:  Air in the pleural space is among the most common etiologies of eosinophilic pleural effusions (EPE). We have recently developed a mouse model of pneumothorax-induced pleural eosinophilia to examine the mechanisms of eosinophil accumulation in pleural tissues. In the present study we examined the significance of IL-5 and IL-13 in

METHODS:  Nine wild-type (wt), 6 IL-5 knockout and 4 IL-13 knockout C57/BL-6 mice where injected intrapleurally with 0.4 ml air. Mice were sacrificed 48 hours after the introduction of pneumothorax because, as we have previously shown, pleural eosinophilia peaks at this time point. Pleural lavage (PL) with 1 ml saline was performed immediately after death. Nucleated cell counts were measured with an automatic cell counter. Cytospins were made and stained with Wright-Giemsa stain for the differential cell count.

RESULTS:  The mean ± SEM PL eosinophil counts were: 1799±188/mm3 in the wt group, 215±53/mm3 in the IL-5 knockout group and 1544±626/mm3 in the IL-13 knockout group. The mean ± SEM PL eosinophil percentages were 33±3.6%, 4.8±1.1% and 26±4.9%, respectively. The PL eosinophil counts and percentages were significantly lower in the IL-5 knockout mice than in the wt (p<0.001, for both the counts and the percentages), or the IL-13 knockout mice (p=0.001, for the percentage and p=0.03 for the counts). However, the PL eosinophil counts and percentages did not differ significantly between wt and IL-13 knockout mice.

CONCLUSION:  IL-5 but not IL-13 is a crucial mediator of pneumothorax-associated pleural eosinophilia.

CLINICAL IMPLICATIONS:  Anti-IL-5 treatment may be useful in the treatment of persistent, symptomatic post-traumatic eosinophilic pleural effusions.

DISCLOSURE:  I.T. Kalomenidis, None.

Monday, October 27, 2003

8:00 AM - 9:30 AM




Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543