0
Articles |

Transgenic Mouse Models To Determine the Role of Epidermal Growth Factor Receptor in Epithelial Cell Proliferation, Apoptosis, and Asbestosis*

Cynthia Timblin, PhD; Raymond Robledo, PhD; Mercedes Rincon, PhD; Andrew Cummins; Linda Pfeiffer; Brooke Mossman, PhD
Author and Funding Information

*From the Departments of Pathology (Drs. Timblin, Robledo, and Mossman, Mr. Cummins, and Ms. Pfeiffer) and Medicine (Dr. Rincon), University of Vermont, Burlington, VT.

Correspondence to: Brooke Mossman, PhD, University of Vermont College of Medicine, Department of Pathology, Medical Alumni Building, Burlington, VT 05405; e-mail: bmossman@zoo.uvm.edu



Chest. 2001;120(1_suppl):S22-S24. doi:10.1378/chest.120.1_suppl.S22
Text Size: A A A
Published online

Extract

Cell proliferation by asbestos fibers is a consequence of early asbestos-induced injury to pulmonary epithelial and mesothelial cells and may be critical to the development of fibroproliferative diseases.12 In mesothelial and alveolar type II epithelial cells, epidermal growth factor (EGF) and crocidolite asbestos are potent mitogens. Asbestos fibers cause aggregation of the EGF receptor (EGFR)3 and increased levels of EGFR steady-state messenger RNA and protein.4 Phosphorylation of the EGFR by asbestos fibers is causally related to increased phosphorylation and activity of extracellular signal-regulated protein kinases (ERKs) 1 and 2 and c-fos protooncogene expression.,45 This pathway may be linked causally to the development of apoptosis and proliferation as increased phosphorylated ERKs are observed in bronchiolar and alveolar type II epithelial cells at sites of developing fibrosis in mice inhaling asbestos fibers.6 These observations suggest that signaling from the EGFR may be related to increased epithelial cell proliferation in the lungs of mice exposed by inhalation to asbestos. To address whether signaling from the EGFR in the pulmonary epithelium is critical to asbestos-induced proliferation and fibrosis, we used transgenic mice expressing a dominant-negative mutant receptor (dnEGFR) under control of the lung-specific, surfactant protein C promoter.

First Page Preview

View Large
First page PDF preview

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Figures

Tables

References

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Sign In to Access Full Content

MEMBER & INDIVIDUAL SUBSCRIBER

Want Access?

NEW TO CHEST?

Become a CHEST member and receive a FREE subscription as a benefit of membership.

Individuals can purchase this article on ScienceDirect.

Individuals can purchase a subscription to the journal.

Individuals can purchase a subscription to the journal or buy individual articles.

Learn more about membership or Purchase a Full Subscription.

INSTITUTIONAL ACCESS

Institutional access is now available through ScienceDirect and can be purchased at myelsevier.com.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Find Similar Articles
CHEST Journal Articles
Pseudomonas aeruginosa-Human Airway Epithelial Cell Interaction*: Effects of Iron on Inflammation and Apoptosis
PubMed Articles
  • CHEST Journal
    Print ISSN: 0012-3692
    Online ISSN: 1931-3543