PURPOSE: Recently, roles of several bacterial species in the pathogenesis and progression of certain infectious diseases have been suggested by detecting and evaluating causative bacteria using molecular biological modalities as cultivation-independent ways. Several reports of detecting bacteria using molecular methods in bronchoalveolar lavage fluids (BALF) in patients with cystic fibrosis (CF) speculated the important roles of anaerobes in the progression of CF. The aim of the present study is evaluating the role of anaerobes in the progression of nontuberculous mycobacteriosis (NTM) by means of bacterial floral analysis in BALF in patients with NTM using the molecular biological method, 16S rRNA gene sequencing analysis.
METHODS: Forty-four patients with the suspicion of NTM by clinical and chest computed tomography findings were enrolled in this study, and 22 cases were diagnosed as NTM. BALF taken from the pathological lesions of these 22 patients were evaluated by both culture-independent and conventional culture methods. Culture-independent method was conducted as follows. Approximately 600 bp of 16SrRNA gene was amplified by polymerase chain reaction using universal primers and then clone library was constructed. Nucleotide sequences of randomly chosen approximately 90 clones for each specimen were determined and the homology with in-house database was searched to determine bacterial species.
RESULTS: Bacterial floral analysis using rRNA gene sequencing method was performed in 18 cases out of 22 NTM patients. High rates (40-90%) of anaerobes (Prevotella, Fusobacterium, Peptostreptococcus species) other than Mycobacterium species were observed in all 18 patients.
CONCLUSIONS: This study revealed that a higher prevalence of anaerobes in BALF from NTM patients.
CLINICAL IMPLICATIONS: Similar to CF, it is speculated that anaerobes may have an important role in the progression of NTM.
DISCLOSURE: The following authors have nothing to disclose: Toshinori Kawanami, Kei Yamasaki, Kazuhiro Yatera, Shuya Nagata, Chinatsu Nishida, Hiroshi Ishimoto, Kazumasa Fukuda, Hatsumi Taniguchi, Hiroshi Mukae
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