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Original Research: LUNG CANCER |

Smoking-Induced Upregulation of AKR1B10 Expression in the Airway Epithelium of Healthy Individuals

Rui Wang, MD, PhD; Guoqing Wang, PhD; Megan J. Ricard, MS; Barbara Ferris, BA; Yael Strulovici-Barel, MSc; Jacqueline Salit, MS; Neil R. Hackett, PhD; Lorraine J. Gudas, PhD; Ronald G. Crystal, PhD
Author and Funding Information

From the Department of Genetic Medicine (Drs R. Wang, G. Wang, Hackett, and Crystal and Mss Ferris, Strulovici-Barel, and Salit), and Department of Pharmacology (Ms Ricard and Dr Gudas), Weill Cornell Medical College, New York, NY.

Correspondence to: Ronald G. Crystal, PhD, Department of Genetic Medicine, Weill Cornell Medical College, 1300 York Ave, Box 96, New York, NY 10065; e-mail: geneticmedicine@med.cornell.edu


Funding/Support: This study was supported, in part, by the National Institutes of Health [R01 HL074326, P50 HL084936, UL1-RR024996, T32 HL094284] and the National Cancer Institute [R01CA097543].

Reproduction of this article is prohibited without written permission from the American College of Chest Physicians (http://www.chestpubs.org/site/misc/reprints.xhtml).


© 2010 American College of Chest Physicians


Chest. 2010;138(6):1402-1410. doi:10.1378/chest.09-2634
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Background:  The aldo-keto reductase (AKR) gene superfamily codes for monomeric, soluble reduced nicotinamide adenine dinucleotide phosphate-dependent oxidoreductases that mediate elimination reactions. AKR1B10, an AKR that eliminates retinals, has been observed as upregulated in squamous metaplasia and non-small cell lung cancer and has been suggested as a diagnostic marker specific to tobacco-related carcinogenesis. We hypothesized that upregulation of AKR1B10 expression may be initiated in healthy smokers prior to the development of evidence of lung cancer.

Methods:  Expression of AKR1B10 was assessed at the mRNA level using microarrays with TaqMan confirmation in the large airway epithelium (21 healthy nonsmokers, 31 healthy smokers) and small airway epithelium (51 healthy nonsmokers, 58 healthy smokers) obtained by fiberoptic bronchoscopy and brushing.

Results:  Compared with healthy nonsmokers, AKR1B10 mRNA levels were significantly upregulated in both large and small airway epithelia of healthy smokers. Consistent with the mRNA data, AKR1B10 protein was significantly upregulated in the airway epithelium of healthy smokers as assessed by Western blot analysis and immunohistochemistry, with AKR1B10 expressed in both differentiated and basal cells. Finally, cigarette smoke extract mediated upregulation of AKR1B10 in airway epithelial cells in vitro, and transfection of AKR1B10 into airway epithelial cells enhanced the conversion of retinal to retinol.

Conclusions:  Smoking per se mediates upregulation of AKR1B10 expression in the airway epithelia of healthy smokers with no evidence of lung cancer. In the context of these observations and the link of AKR1B10 to the metabolism of retinals and to lung cancer, the smoking-induced upregulation of AKR1B10 may be an early process in the multiple events leading to lung cancer.

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