PURPOSE: To investigate the effect of SiRNA-Ku70 in drug-resistance in A549DDP cell line.
METHODS: Expression of apoptosis-related genes was examined by PCR-Array assay.
RESULTS: Expression of BAX,P53 and P73 increased significantly whereas the expression of TRAF and BFAR decreased in SiRNA-Ku70 A549DDP cells compared to A549DDP cells (P< 0.05) .
CONCLUSION: These results show that Ku70 might play an important role in drug-resistance in A549DDP cell line. Silencing of Ku70 can be attributed to the elevation of apoptotic genes- BAX, P53 and P73 expression; reduction of anti-apoptotic genes TRAF and BFAR. This study suggests that silencing of Ku70 might reverse the drug-resistance in A549DDP cell line.
CLINICAL IMPLICATIONS: Ku70 might be a new target for the drug-resistant reversion.
DISCLOSURE: Jie Zhang, No Financial Disclosure Information; No Product/Research Disclosure Information