PURPOSE: To study the interaction of nontypeable haemophilus influenzae strain ATCC49247 with primary human bronchial epithelial cells (HBEC) and the NTHi-induced release and expression of proinflammoraty cytokine in the HBEC.
METHODS: HBEC were isolated by using low temperature protease digestion and cultured in serum-free medium. Confluent epithelial cell cultures were incubated with NTHi, NTHi +erythromycin(0.1mg/L), NTHi + erythromycin(10mg/L), NTHi + gentamicin(100mg/L), NTHi+dexamethasone(100uM)and normal untreated control cells after 24h. Release of IL-8 and TNF- into the supernatant was assayed by enzyme-linked immunosorbent assay; The expression of ICAM-1 was examined by immunohistochemistry staining.
RESULTS: (1) Some HBECs were transformed and died after 24h. (2) HBEC: NTHi-induced cells released significantly greater amounts of IL-8 and TNF- (2172.18131.83, 7.222.17 pg/ml, respectively) than normal untreated cells (1115.7657.63,2.841.04pg/ml)(p< 0.001). NTHi also significantly increased the total number of of ICAM-1 positive cells from 105%(in control untreated cultures) to 805%(p< 0.001). Similarly, incubation of HBEC with 0.1mg/L erythromycin and gentamicin significantly induced release of IL-8, TNF-a and the expression of ICAM-1, which was blocked by 10mg/L erythromycin and 100uM dexamethasone.
CONCLUSION: HBEC can release IL-8 and TNF-. NTHi may increase significantly release and expression of proinflammatory cytokine. Gentamicin have no anti-inflammatory effects. Erythromycin may have anti-inflammatory effects. Dexamethasone has distinct anti-inflammatory effects.
CLINICAL IMPLICATIONS: this study indicated that during infection there may be necessarities to add some anti-inflammation drugs.
DISCLOSURE: Guifang Wang, No Financial Disclosure Information; No Product/Research Disclosure Information