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Poster Presentations: Wednesday, November 3, 2010 |

Study on Human Tracheal Bronchial Epithelial Cells Secreting Cytokines in Vitro by Nontypeable Haemophilus Influenzae FREE TO VIEW

Jianmei Qian, MD; Chunxue Bai, MD; Guifang Wang
Author and Funding Information

zhongshan hospital, Shanghai, Peoples Rep of China



Chest. 2010;138(4_MeetingAbstracts):599A. doi:10.1378/chest.10615
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Abstract

PURPOSE: To study the interaction of nontypeable haemophilus influenzae strain ATCC49247 with primary human bronchial epithelial cells (HBEC) and the NTHi-induced release and expression of proinflammoraty cytokine in the HBEC.

METHODS: HBEC were isolated by using low temperature protease digestion and cultured in serum-free medium. Confluent epithelial cell cultures were incubated with NTHi, NTHi +erythromycin(0.1mg/L), NTHi + erythromycin(10mg/L), NTHi + gentamicin(100mg/L), NTHi+dexamethasone(100uM)and normal untreated control cells after 24h. Release of IL-8 and TNF- into the supernatant was assayed by enzyme-linked immunosorbent assay; The expression of ICAM-1 was examined by immunohistochemistry staining.

RESULTS: (1) Some HBECs were transformed and died after 24h. (2) HBEC: NTHi-induced cells released significantly greater amounts of IL-8 and TNF- (2172.18131.83, 7.222.17 pg/ml, respectively) than normal untreated cells (1115.7657.63,2.841.04pg/ml)(p< 0.001). NTHi also significantly increased the total number of of ICAM-1 positive cells from 105%(in control untreated cultures) to 805%(p< 0.001). Similarly, incubation of HBEC with 0.1mg/L erythromycin and gentamicin significantly induced release of IL-8, TNF-a and the expression of ICAM-1, which was blocked by 10mg/L erythromycin and 100uM dexamethasone.

CONCLUSION: HBEC can release IL-8 and TNF-. NTHi may increase significantly release and expression of proinflammatory cytokine. Gentamicin have no anti-inflammatory effects. Erythromycin may have anti-inflammatory effects. Dexamethasone has distinct anti-inflammatory effects.

CLINICAL IMPLICATIONS: this study indicated that during infection there may be necessarities to add some anti-inflammation drugs.

DISCLOSURE: Guifang Wang, No Financial Disclosure Information; No Product/Research Disclosure Information

12:45 PM - 2:00 PM


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