Slide Presentations: Monday, November 1, 2010 |

Effects of Cigarette Smoking on Expression of Matrix Metalloproteinase-12 in Airway Epithelium of Rats FREE TO VIEW

Yi Ming Yuan, MD; Xiao-Yan Zhang, RN; Ming Yang, MD
Author and Funding Information

West China Hospital Sichuan University, Chengdu, Peoples Rep of China

Chest. 2010;138(4_MeetingAbstracts):750A. doi:10.1378/chest.10056
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PURPOSE: To explore the effects of smoking intensity,duration and cessation on mRNA and protein expression of matrix metalloproteinase-12(MMP-12) in airway epithelium of rats,and its implication in the pathogenesis of chronic obstructive pulmonary disease(COPD).

METHODS: Forty Wistar rats were randomly divided into five group(control group,long-term heavy smoking group,short-term heavy smoking group,long-term light smoking group and smoking cessation group which was exposed to room air for 10 weeks after long-term heavy smoking).The expression of MMP-12 mRNA and protein in airway epithelium was detected by in situ hybridization and immunohistochemistry respectively.

RESULTS: Emphysema was seen in the lung tissue of every smoking rat,and was most severe in the long-term smoking group.Compared with control group,expression of MMP-12 in airway epithelium was remarkablely elevated in the long-term heavy smoking group, short-term heavy smoking group,long-term light smoking group and smoking cessation group(p<0.01). MMP-12 expression levels in long-term heavy smoking group were higher than that in other three smoking group(p<0.05).

CONCLUSION: Cigarette smoking is related to increased expression of MMP-12 in rats lung tissue.MMP-12 may play an important role in lung injury and airway remodeling in COPD.

CLINICAL IMPLICATIONS: This study provides new evidence supporting the hypothesis that imbalance in proteinase-antiproteinase play an important role in the pathogenesis of COPD.Targeting MMP-12 may provide novel treatment of COPD.

DISCLOSURE: Yi Ming Yuan, No Financial Disclosure Information; No Product/Research Disclosure Information

2:30 PM - 3:45 PM




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