PURPOSE: In the present study the aim was to evaluate the in vitro spontaneous apoptosis in activated peripheral PMN from patients with active, latent TB and healthy PPD skin test negative control subjects, and to determine whether PPD affect the apoptosis in these cells.
METHODS: PMN from PPD-positive control subjects and from patients with active and latent TB were exposed to PPD in culture. Apoptosis of PMN was evaluated by microscopy and DNA content at 0, 3, and 18 h.
RESULTS: Spontaneous apoptosis was accelerated in TB-PMN at 3 h, but no differences were observed in latent TB- or N-PMN at 18 h of culture. When stimulated with PPD, both N-PMN and latent PMN accelerated apoptosis at 3 and 18h. By contrast, MTB delayed the apoptotic rate of active TB-PMN at 3 h, whereas it accelerated apoptosis at 18h.
CONCLUSION: Taken together, these data suggest that the acceleration of apoptosis observed in active TB-PMN could be associated with MTB-induced activation and this might be a point of differentiation between active and latent TB. However, this point needs further confirmation at the site of infection.
CLINICAL IMPLICATIONS: Peripheral Blood Polymorphnuclear Neutrophils Apoptosis may be a new good differentiating point between active and latent tuberculosis.
DISCLOSURE: Amal Fathy Mostafa, No Financial Disclosure Information; No Product/Research Disclosure Information